Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.jprot.2020.103648 Sudipa Maity 1 , Aadil Hussain Bhat 1 , Kuldeep Giri 1 , Kiran Ambatipudi 1
|
Bovine milk has become an important biological fluid for proteomic research due to its nutritional and immunological benefits. To date, over 300 publications have reported changes in bovine milk protein composition based on seasons, lactation stages, breeds, health status and milk fractions while there are no reports on consolidation or overlap of data between studies. Thus, we have developed a literature-based, manually curated open online database of bovine milk proteome, BoMiProt (http://bomiprot.org), with over 3100 proteins from whey, fat globule membranes and exosomes. Each entry in the database is thoroughly cross-referenced including 397 proteins with well-defined information on protein function, biochemical properties, post-translational modifications and significance in milk from different publications. Of 397 proteins, over 199 have been reported with a structural gallery of homology models and crystal structures in the database. The proteome data can be retrieved using several search parameters such as protein name, accession IDs, FASTA sequence. Furthermore, the proteome data can be filtered based on milk fractions, post-translational modifications and/or structures. Taken together, BoMiProt represents an extensive compilation of bovine milk proteins from literature, providing a foundation for future studies to identify specific milk proteins which may be linked to mammary gland pathophysiology.
Biological significance
Protein data identified from different previously published proteomic studies on bovine milk samples (21 publications) were gathered in the BoMiProt database. Unification of the identified proteins will give researchers an initial reference database on bovine milk proteome to understand the complexities of milk as a biological fluid. BoMiProt has a user-friendly interface with several useful features, including different search criteria for primary and secondary information of proteins along with cross-references to external databases. The database will provide insights into the existing literature and possible future directions to investigate further and improve the beneficial effects of bovine milk components and dairy products on human health.
中文翻译：
BoMiProt：牛乳蛋白数据库。
牛乳由于其营养和免疫学优势，已成为蛋白质组学研究的重要生物液体。迄今为止，已有300多家出版物报道了牛乳蛋白组成随季节，泌乳阶段，品种，健康状况和乳汁成分的变化而变化，但尚无研究之间数据合并或重叠的报道。因此，我们建立了一个基于文献的，手动管理的，开放式的在线数据库，其中包含牛乳蛋白质组BoMiProt（http://bomiprot.org），其中包含来自乳清，脂肪小球膜和外泌体的3100多种蛋白质。数据库中的每个条目都经过全面的交叉引用，包括397种蛋白质，以及来自不同出版物的关于蛋白质功能，生化特性，翻译后修饰和牛奶中重要性的明确信息。在397种蛋白质中，据报道，已有199多个数据库具有同源模型和晶体结构的结构库。可以使用几个搜索参数来检索蛋白质组数据，例如蛋白名称，登录号，FASTA序列。此外，可以基于乳汁成分，翻译后修饰和/或结构来过滤蛋白质组数据。综上所述，BoMiProt代表了文献中对牛乳蛋白的广泛汇编，为将来的研究确定可能与乳腺病理生理学相关的特定乳蛋白奠定了基础。蛋白质组数据可以根据乳汁成分，翻译后修饰和/或结构进行过滤。综上所述，BoMiProt代表了文献中对牛乳蛋白的广泛汇编，为将来的研究确定可能与乳腺病理生理学相关的特定乳蛋白奠定了基础。蛋白质组数据可以根据乳汁成分，翻译后修饰和/或结构进行过滤。综上所述，BoMiProt代表了文献中对牛乳蛋白的广泛汇编，为将来的研究确定可能与乳腺病理生理学相关的特定乳蛋白奠定了基础。
生物学意义
在BoMiProt数据库中收集了从先前发表的有关牛乳样品的不同蛋白质组学研究（21种出版物）中鉴定出的蛋白质数据。鉴定出的蛋白质的统一将为研究人员提供有关牛乳蛋白质组的初步参考数据库，以了解牛奶作为生物液体的复杂性。BoMiProt具有易于使用的界面，具有多种有用的功能，包括针对蛋白质一级和二级信息的不同搜索条件以及对外部数据库的交叉引用。该数据库将提供对现有文献的见识以及可能的未来方向，以进一步调查并改善牛乳成分和乳制品对人体健康的有益影响。
京公网安备 11010802027423号