Abstract A molecularly imprinted polymer was synthesized on the surface of magnetic nanoparticles for subsequent application in the extraction of trimethoprim (TMT) and sulfamethoxazole (SMX) residues in bovine milk. The magnetic polymer was suitably characterized and demonstrated high stability and optimum adsorption capacity under the conditions of analysis. MMIP was used in the preparation of milk samples by the magnetic solid phase extraction technique whose optimized parameters were: sample volume, elution solvent and its volume, amount of adsorbent, stirring time, and pH of the sample. The relative recoveries of analytes were 98.66 ± 5.59% and 75.57 ± 0.72% for TMT and SMX, respectively. The method developed was linear in the range of concentration of 0.05–20 µg mL−1 with correlation coefficients of 0.997 and 0.994 for TMT and SMX, respectively. It was also demonstrated satisfactory accuracy and precision, as well as adequate robustness and good drugs stability in the matrix. The method was applied satisfactorily in real milk samples contaminated with residues of these antibiotics.

中文翻译：

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用于选择性测定牛奶中甲氧苄啶和磺胺甲恶唑的磁性分子印迹聚合物的高效开发

摘要 在磁性纳米粒子表面合成了分子印迹聚合物，用于提取牛乳中甲氧苄啶（TMT）和磺胺甲恶唑（SMX）残留。在分析条件下，磁性聚合物被适当地表征并显示出高稳定性和最佳吸附能力。MMIP用于通过磁性固相萃取技术制备牛奶样品，其优化参数为：样品体积、洗脱溶剂及其体积、吸附剂用量、搅拌时间和样品pH。TMT 和 SMX 分析物的相对回收率分别为 98.66 ± 5.59% 和 75.57 ± 0.72%。开发的方法在 0.05–20 µg mL-1 的浓度范围内呈线性，TMT 和 SMX 的相关系数分别为 0.997 和 0.994，分别。它还被证明具有令人满意的准确度和精密度，以及足够的稳定性和基质中良好的药物稳定性。该方法在被这些抗生素残留物污染的真实牛奶样品中得到了令人满意的应用。