Abstract An efficient amplification strategy based on the G-triplex DNA structure was developed for label- and enzyme-free fluorescence detection of human immunodeficiency virus (HIV) DNA. In this system, three hairpin molecules (H1, H2, and H3) were used to detect the target and amplify the signal. Fluorescence enhancement was achieved by producing more G-triplexes to bind with Thioflavine T. A detection limit of 33 pM with good linearity in the range from 400 pM to 110 nM was observed. This method avoided using of enzyme and cumbersome labeling procedures and was relatively superior to previous G-quadruplex-based approaches, which often encounter some difficulties in control and excitation. Moreover, the application for detecting HIV-DNA in biological fluids indicates that further applications for clinical diagnosis are expected.

中文翻译：

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使用 G-三链体作为信号报告基因，在循环 DNA 自组装策略上设计无标记和无酶检测 HIV-DNA

摘要 开发了一种基于 G-三链体 DNA 结构的高效扩增策略，用于人类免疫缺陷病毒 (HIV) DNA 的无标记和无酶荧光检测。在该系统中，三个发夹分子（H1、H2 和 H3）用于检测目标并放大信号。通过产生更多的 G-三链体与硫黄素 T 结合来实现荧光增强。观察到 33 pM 的检测限，在 400 pM 到 110 nM 的范围内具有良好的线性。这种方法避免了使用酶和繁琐的标记程序，并且相对优于以前的基于 G-四链体的方法，后者在控制和激发方面经常遇到一些困难。此外，检测生物体液中 HIV-DNA 的应用表明有望进一步应用于临床诊断。