Some Trichoderma spp. have an ability to inhibit proliferation of fungal plant pathogens in the soil. Numerous compounds with a proven antifungal activity are synthesized via the terpene pathway. Here, we stimulated the activity of the mevalonate pathway in T. atroviride P1 by expressing the Saccharomyces cerevisiae ERG20 gene coding for farnesyl pyrophosphate (FPP) synthase, a key enzyme of this pathway. ERG20-expressing Trichoderma strains showed higher activities of FPP synthase and squalene synthase, the principal recipient of FPP in the mevalonate pathway. We also observed activation of dolichyl phosphate mannose (DPM) synthase, an enzyme in protein glycosylation, and significantly increased O- and N-glycosylation of secreted proteins. The hyper-glycosylation of secretory hydrolases could explain their increased activity observed in the ERG20 transformants. Analysis of the antifungal properties of the new strains revealed that the hydrolases secreted by the transformants inhibited growth of a plant pathogen, Pythium ultimum more efficiently compared to the control strain. Consequently, the biocontrol activity of the transgenic strains, determined as their ability to protect bean seeds and seedlings against harmful action of P. ultimum, was also improved substantially.

中文翻译：

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甲羟戊酸途径的固醇分支的活性增加可提高分泌蛋白的糖基化，并改善木霉阿魏病毒的抗真菌特性。

一些木霉属。具有抑制真菌植物病原体在土壤中繁殖的能力。具有公认的抗真菌活性的许多化合物都是通过萜烯途径合成的。在这里，我们通过表达编码法呢基焦磷酸酯（FPP）合酶的酿酒酵母ERG20基因（该途径的关键酶），刺激了阿曲韦杆菌P1中甲羟戊酸途径的活性。表达ERG20的木霉菌株显示FPP合酶和角鲨烯合酶的活性较高，这是甲羟戊酸途径中FPP的主要受体。我们还观察到了磷酸二氢甘油磷酸甘露糖（DPM）合酶（一种蛋白质糖基化酶）的激活，并显着增加了分泌蛋白质的O-和N-糖基化。分泌水解酶的高糖基化可以解释其在ERG20转化子中观察到的活性增加。对新菌株的抗真菌特性的分析表明，与对照菌株相比，转化子分泌的水解酶更有效地抑制了植物病原体终生腐霉的生长。因此，转基因菌株的生防活性也被显着提高，该转基因菌株被确定为它们保护豆种子和幼苗免受P. ultimum有害作用的能力。