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Utilization of High throughput microcrystalline cellulose decorated silver nanoparticles as an eco-nematicide on root-knot nematodes.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.colsurfb.2020.110805
Moustafa M G Fouda 1 , Nader R Abdelsalam 2 , I M A Gohar 3 , Amira E M Hanfy 2 , Sarah I Othman 4 , Amera F Zaitoun 2 , Ahmed A Allam 5 , Osama M Morsy 6 , Mehrez El-Naggar 1
Affiliation  

The present study aimed to evaluate the influence of high throughput microcrystalline cellulose embedded silver nanoparticles (Ag-NPs), as an alternative eco-nematicide on Root-knot nematode (Meliodogyne incognita), which deem the main reason toward the loss of more than 20% in crops worldwide. In this work, Ag-NPs was prepared in very high concentration. Ag-NPs prepared using such technique has many advantages such as: absence of organic or solvents, scaling up thru using high concentration of silver precursor and utilization of environmentally benign polymer; Microcrystalline Cellulose (MCC). At the beginning, the bulk Ag-NPs colloidal solution is diluted to 5, 10, 15, 20, 25, 30, 40, 50, 60, 75, 80 and 100 ppm. Then, heavily galled roots of annual seed-propagated weed, Solanum nigrum L. family Solanaceae were selected to identify the Meloidogyne species and followed by treatment with the previously Ag-NPs concentrations. Results obtained after 24 h incubation, showed the highest mortality (M%) (40.36 ± 1.15%) which was achieved by means of 20 ppm of Ag-NPs compared with the highest concentration of Ag-NPs; 100 ppm (42.85 ± 3.51%). It was obviously noticed that, by increasing the concentration of Ag-NPs, M % decreased. On the other hand, after 48 h, 30 ppm Ag-NPs showed the highest M%; (52.82 ± 0.57%), while, after 72 h of treatments, the M% reached 95.53 ± 0.57% using 40 ppm Ag-NPs, then decreased to 66.67 ± 2.00% using 100 ppm Ag-NPs. All previous finding affirms the effectiveness of lower concentrations of Ag-NPs compared with the highest one, after 72 h. In conclusion, Ag-NPs could be successfully used as eco-nematicide for Root-knot nematodes; Meloidogyne incognita with a recommended dose of 20-40 ppm that is acquired higher M% and caused many aberrations during the different growth stages.

中文翻译:

利用高通量微晶纤维素修饰的银纳米颗粒作为根结线虫上的生态杀线虫剂。

本研究旨在评估高通量微晶纤维素包埋的银纳米颗粒(Ag-NPs)作为根结线虫(Meliodogyne incognita)的替代生态杀线虫剂的影响,认为这是造成20多株损失的主要原因全世界农作物的百分比。在这项工作中,制备了非常高浓度的Ag-NPs。用这种技术制备的Ag-NP具有许多优点,例如:不存在有机物或溶剂,使用高浓度的银前体扩大规模并利用对环境无害的聚合物;微晶纤维素(MCC)。首先,将大量的Ag-NPs胶体溶液稀释至5、10、15、15、20、25、30、40、50、60、75、80和100 ppm。然后,一年生种子繁殖杂草Solanum nigrum L的根部积gall了很多。选择茄科(Solanaceae)来鉴定根结线虫种类,然后用先前的Ag-NPs浓度处理。孵育24小时后获得的结果显示,与最高浓度的Ag-NP相比,通过20 ppm的Ag-NPs可获得最高的死亡率(M%)(40.36±1.15%)。100 ppm(42.85±3.51%)。显然注意到,通过增加Ag-NP的浓度,M%降低。另一方面,48小时后,30 ppm Ag-NPs表现出最高的M%。(52.82±0.57%),而在处理72小时后,使用40 ppm Ag-NPs的M%达到95.53±0.57%,然后使用100 ppm Ag-NPs降低到66.67±2.00%。所有先前的发现都证实,在72小时后,与最高浓度的Ag-NPs相比,较低浓度的Ag-NPs的有效性。结论,Ag-NPs可以成功地用作根结线虫的杀线虫剂。建议的剂量为20-40 ppm的根结线虫(Meloidogyne incognita)获得更高的M%,并在不同的生长阶段引起许多像差。
更新日期:2020-01-17
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