Preparation and application of PGMA-DVB microspheres via surface-modification with quaternary and phenylboronic acid moiety.,Colloids and Surfaces B: Biointerfaces

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Preparation and application of PGMA-DVB microspheres via surface-modification with quaternary and phenylboronic acid moiety.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.colsurfb.2020.110807
Long Pang ₁ , Tingting Xue ₁ , Hailin Cong ₂ , Youqing Shen ₁ , Bing Yu ₂

Affiliation

Porous cross-linked poly (glycidyl methacrylate-divinylbenzene) (PGMA-DVB) particles (7.42 ± 0.24 μm in diameter) were prepared by an improved two-step seed swelling polymerization method. The PGMA-DVB particles were further modified with poly (allylamine hydrochloride) (PAH) and 4-(bromomethyl) phenylboronic acid (BPA), which were used as high performance liquid chromatography (HPLC) filler for nucleotides separation. The liquid chromatographic column packing materials successfully achieved complete separation of nucleotides mixture or deoxynucleotides mixture based on non-polar adsorption, hydrogen bonding interaction and electrostatic adsorption. Reckoning on the chemical structure of BPA, nucleotides and deoxynucleotides with same base group were also separated. The column packing materials were durable after over 100 time running or 7 days. It presents a kind of new notion for the separation of nucleotides by HPLC.

中文翻译：

PGMA-DVB微球的制备及其季铵和苯基硼酸部分的表面改性。

通过改进的两步种子溶胀聚合法制备了多孔交联的聚甲基丙烯酸缩水甘油酯-二乙烯基苯（PGMA-DVB）颗粒（直径7.42±0.24μm）。PGMA-DVB颗粒进一步用聚烯丙胺盐酸盐（PAH）和4-（溴甲基）苯基硼酸（BPA）改性，它们用作高效液相色谱（HPLC）填料用于核苷酸分离。液相色谱柱填充材料基于非极性吸附，氢键相互作用和静电吸附，成功实现了核苷酸混合物或脱氧核苷酸混合物的完全分离。根据双酚A的化学结构，还分离了具有相同碱基的核苷酸和脱氧核苷酸。经过100多次运行或7天后，色谱柱填充材料是耐用的。

更新日期：2020-01-17

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