The present study explores the rapid chlopyrifos (CPs) biodegradation potential of plant growth promoting (PGP) psychrophilic bacteria isolated from brackish water by enrichment culture technique. Based on biochemical tests and 16 S rDNA sequencing the isolate was identified as Shewanella sp. The isolate Shewanella BT05 showed significant growth rate in various concentrations of (10-50 mg/L) CPs. The isolate produced plant growth promoting factors, IAA (20.8 ± 1.2 and 15.4 ± 1.0 μg/mL) and siderophores (60.67 ± 1.2 and 57.5 ± 0.9%) in the absence and presence of CPs. Further, the isolate BT05 solublized phosphate (16.5 ± 1.0 and 12.0 ± 1.0 mm in size respectively), and produce hydrogen cyanide (excellent and moderate) in the presence and absence of CPs. The isolate BT05 degraded 94.3, 91.8, 87.9, 82.6, and 80.5% of CPs at 10, 20, 30, 40, and 50 mg/L, respectively, within 24 h. Further, the media conditions were optimized for enhanced CPs removal and observed 93% removal in the presence of 3.5% glucose in pH 7.0 at 32.5 °C. Fourier-transform infrared spectroscopy and high-performance liquid chromatography results indicated the role for Shewanella BT05 in the biomineralization of CPs. The results suggested the isolate BT05 could be used for CPs removal as well as PGP activity in contaminated soil.

中文翻译：

---

通过促进植物生长的嗜冷性希瓦氏菌属物种对毒死rif进行快速生物降解。BT05：一种清洁农药污染环境的环保方法。

本研究探索了通过富集培养技术从微咸水中分离出的植物促生长（PGP）嗜冷细菌的快速毒死y（CPs）生物降解潜力。根据生化测试和16 S rDNA测序，分离物被鉴定为Shewanella sp。分离株希瓦氏菌BT05在各种浓度（10-50 mg / L）CPs中均显示出显着的生长速率。在没有CP的情况下，该分离物产生了植物生长促进因子IAA（20.8±1.2和15.4±1.0μg/ mL）和铁载体（60.67±1.2和57.5±0.9％）。此外，分离物BT05溶解了磷酸盐（尺寸分别为16.5±1.0和12.0±1.0 mm），并在有无CP的情况下产生氰化氢（优异和中等）。分离株BT05在10、20、30、40，在24小时内分别达到50 mg / L和50 mg / L。此外，优化了培养基条件以提高CP的去除率，并在32.5°C下在pH 7.0中存在3.5％葡萄糖的条件下观察到93％去除率。傅里叶变换红外光谱和高效液相色谱结果表明，希瓦氏菌BT05在CP的生物矿化中发挥了作用。结果表明，分离物BT05可用于去除污染土壤中的CP和PGP活性。