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Zwitterionic Polypeptides: Chemoenzymatic Synthesis and Loosening Function for Cellulose Crystals.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-01-24 , DOI: 10.1021/acs.biomac.9b01700 Kousuke Tsuchiya 1 , Neval Yilmaz 1 , Takaaki Miyamoto 1 , Hiroyasu Masunaga 2 , Keiji Numata 1
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-01-24 , DOI: 10.1021/acs.biomac.9b01700 Kousuke Tsuchiya 1 , Neval Yilmaz 1 , Takaaki Miyamoto 1 , Hiroyasu Masunaga 2 , Keiji Numata 1
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A polypeptide with a GlyHisGly repeating sequence containing zwitterionic structures that effectively interact with cellulose was synthesized for dissociation of cellulose crystals. Polypeptide with the GlyHisGly sequence was synthesized by chemoenzymatic polymerization and postfunctionalization of the His residues was performed to afford imidazolium butyrate on the side chains. The resulting zwitterionic polypeptide effectively dissociated bundles of tunicate cellulose nanocrystals, even when the conditions were mild and the concentration of the polypeptide was as low as 1-2 mg mL-1. Polypeptide treatment also affected the morphology of the cell walls in cultured plant cells, and the cellulose microfibril networks and amorphous polysaccharide layer were dissociated according to atomic force microscopy (AFM). The zwitterionic polypeptide treatment did not change the crystal structure of the cellulose nanocrystals. Analysis of the mechanical properties of the cellulose nanocrystals by force curve measurements using AFM revealed that the elastic modulus of the cellulose nanocrystals increased after treatment with the zwitterionic polypeptide, indicating that the amorphous part of the cellulose nanocrystals was removed by interactions with the polypeptide. At a concentration of the polypeptide that enabled the dissociation of the cellulose network, the zwitterionic polypeptide showed negligible cytotoxicity to the plant cells. The mild and noncytotoxic technique for loosening cellulose microfibrils/nanocrystals that was developed in this study has tremendous significance for the modification of cellulose in terms of polymer chemistry, material science, and plant biotechnology.
中文翻译：
两性离子多肽：纤维素晶体的化学酶法合成和疏松功能。
合成具有GlyHisGly重复序列的多肽，该序列包含与纤维素有效相互作用的两性离子结构，用于纤维素晶体的解离。通过化学酶促聚合合成具有GlyHisGly序列的多肽，并进行His残基的后功能化以在侧链上提供丁酸咪唑鎓。即使当条件温和且多肽的浓度低至1-2 mg mL-1时，所得两性离子多肽也能有效解离束缚纤维素纳米晶体束。多肽处理还影响培养的植物细胞中细胞壁的形态，并且根据原子力显微镜（AFM）将纤维素微原纤维网络和无定形多糖层解离。两性离子多肽处理不会改变纤维素纳米晶体的晶体结构。通过使用AFM的力曲线测量对纤维素纳米晶体的机械性能进行分析，结果表明，用两性离子多肽处理后，纤维素纳米晶体的弹性模量增加，表明纤维素纳米晶体的无定形部分通过与多肽的相互作用而被除去。在能够使纤维素网络解离的多肽浓度下，两性离子多肽对植物细胞的细胞毒性可忽略不计。这项研究开发的温和无细胞毒性的纤维素微纤维/纳米晶体松弛技术，对高分子化学，材料科学，
更新日期：2020-01-16
中文翻译：
两性离子多肽：纤维素晶体的化学酶法合成和疏松功能。
合成具有GlyHisGly重复序列的多肽，该序列包含与纤维素有效相互作用的两性离子结构，用于纤维素晶体的解离。通过化学酶促聚合合成具有GlyHisGly序列的多肽，并进行His残基的后功能化以在侧链上提供丁酸咪唑鎓。即使当条件温和且多肽的浓度低至1-2 mg mL-1时，所得两性离子多肽也能有效解离束缚纤维素纳米晶体束。多肽处理还影响培养的植物细胞中细胞壁的形态，并且根据原子力显微镜（AFM）将纤维素微原纤维网络和无定形多糖层解离。两性离子多肽处理不会改变纤维素纳米晶体的晶体结构。通过使用AFM的力曲线测量对纤维素纳米晶体的机械性能进行分析，结果表明，用两性离子多肽处理后，纤维素纳米晶体的弹性模量增加，表明纤维素纳米晶体的无定形部分通过与多肽的相互作用而被除去。在能够使纤维素网络解离的多肽浓度下，两性离子多肽对植物细胞的细胞毒性可忽略不计。这项研究开发的温和无细胞毒性的纤维素微纤维/纳米晶体松弛技术，对高分子化学，材料科学，
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