Cardiac tissue engineering is of particular importance in the combination of contracting cells with a biomaterial scaffold, which serves as a cell-delivery construct, to replace cardiomyocytes (CMs) that are lost as a result of an infarction, to restore heart function. However, most biomaterial scaffolds are nonconductive and may delay regional conduction, potentially causing arrhythmias. In this study, a conductive CM-delivery construct that consists of a gelatin-based gelfoam that is conjugated with a self-doped conductive polymer (poly-3-amino-4-methoxybenzoic acid, PAMB) is proposed as a cardiac patch (PAMB-Gel patch) to repair an infarcted heart. A nonconductive plain gelfoam (Gel patch) is used as a control. The electrical conductivity of the PAMB-Gel patch is approximately 30 times higher than that of the Gel patch; as a result, the conductive PAMB-Gel patch can substantially increase electrical conduction between distinct clusters of beating CMs, facilitating their synchronous contraction. In vivo epicardial implantation of the PAMB-Gel patch that is seeded with CMs (the bioengineered patch) in infarcted rat hearts can significantly enhance electrical activity in the fibrotic tissue, improving electrical impulse propagation and synchronizing CM contraction across the scar region, markedly reducing its susceptibility to cardiac arrhythmias. Echocardiography shows that the bioengineered conductive patch has an important role in the restoration of cardiac function, perhaps owing to the synergistic effects of its conductive construct and the synchronously beating CMs. These experimental results reveal that the as-proposed bioengineered conductive patch has great potential for repairing injured cardiac tissues.

中文翻译：

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导电细胞递送构建体，是一种生物工程补丁，可以改善电传播并同步心肌细胞收缩，以修复心脏。

在将收缩细胞与生物材料支架相结合时，心脏组织工程特别重要，该支架用作细胞输送结构，以替代因梗塞而丢失的心肌细胞（CM），以恢复心脏功能。但是，大多数生物材料支架是不导电的，可能会延迟区域传导，从而可能导致心律不齐。在这项研究中，提出了一种由以明胶为基础的明胶泡沫与自掺杂导电聚合物（聚-3-氨基-4-甲氧基苯甲酸，PAMB）缀合而成的导电CM传递构建体-凝胶贴片）以修复梗塞的心脏。非导电的普通明胶泡沫（凝胶贴剂）用作对照。PAMB-Gel贴剂的电导率大约是Gel贴剂的电导率的30倍；结果是，导电的PAMB-Gel贴片可显着增加搏动CM的不同簇之间的导电性，促进其同步收缩。在心肌梗死的大鼠心脏内植入植入有CM的PAMB-Gel贴剂（生物工程贴剂），可以显着增强纤维化组织中的电活动，改善电脉冲的传播并同步化CM在整个疤痕区域的收缩，从而显着降低其疤痕区域。对心律不齐的敏感性。超声心动图显示，生物工程导电贴片在恢复心脏功能中具有重要作用，这可能是由于其导电构造和同步跳动的CM的协同作用。