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Controlled release and antioxidant activity of chitosan and β-lactoglobulin complex nanoparticles loaded with epigallocatechin gallate.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-16 , DOI: 10.1016/j.colsurfb.2020.110802 Wenzhong Dai 1 , Chengcheng Ruan 1 , Yue Sun 1 , Xueling Gao 2 , Jin Liang 1
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-16 , DOI: 10.1016/j.colsurfb.2020.110802 Wenzhong Dai 1 , Chengcheng Ruan 1 , Yue Sun 1 , Xueling Gao 2 , Jin Liang 1
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In order to investigate the influence of different embedding methods on the properties and function of polyphenols, the Epigallocatechin gallate (EGCG) loaded chitosan nanoparticles prepared with or without β-lactoglobulin (β-Lg) were obtained by ionic cross linking method. The average particle sizes of EGCG loaded chitosan nanoparticles (EGCG-CS NPs) decreased from 190 nm to 157 nm after adding with β-Lg, whereas the encapsulation efficiency (EE) increased from 59.79 % to 76.29 %. The results of transmission electron microscopy (TEM) showed that the obtained nanoparticles had obvious core-shell structure. The results of simulated gastrointestinal digestion showed that the release rate of EGCG in CS/β-Lg NPs was much lower than that of CS-NPs. Compared with free EGCG, the DPPH and FRAP assay showed that EGCG-CS NPs and EGCG-CS/β-Lg NPs had slow-controlled antioxidant activity. Meanwhile, the study of cellular antioxidant activity (CAA) showed that the EC50 values of EGCG-CS NPs and EGCG-CS/β-Lg NPs were decreased by 8.56 % and 18.35 %, respectively.
中文翻译：
负载表没食子儿茶素没食子酸酯的壳聚糖和β-乳球蛋白复合纳米颗粒的控释和抗氧化活性。
为了研究不同包埋方法对多酚性质和功能的影响，通过离子交联法获得了含或不含β-乳球蛋白（β-Lg）的表没食子儿茶素没食子酸酯（EGCG）负载的壳聚糖纳米颗粒。加入β-Lg后，负载EGCG的壳聚糖纳米颗粒（EGCG-CS NPs）的平均粒径从190 nm减小到157 nm，而包封效率（EE）从59.79％增加到76.29％。透射电子显微镜（TEM）的结果表明，所得纳米颗粒具有明显的核-壳结构。模拟胃肠道消化的结果表明，CS /β-LgNPs中EGCG的释放速率远低于CS-NPs。与免费的EGCG相比，DPPH和FRAP分析表明EGCG-CS NPs和EGCG-CS /β-LgNPs具有缓慢控制的抗氧化活性。同时，对细胞抗氧化活性（CAA）的研究表明，EGCG-CS NPs和EGCG-CS /β-LgNPs的EC50值分别降低了8.56％和18.35％。
更新日期：2020-01-16
中文翻译：
负载表没食子儿茶素没食子酸酯的壳聚糖和β-乳球蛋白复合纳米颗粒的控释和抗氧化活性。
为了研究不同包埋方法对多酚性质和功能的影响，通过离子交联法获得了含或不含β-乳球蛋白（β-Lg）的表没食子儿茶素没食子酸酯（EGCG）负载的壳聚糖纳米颗粒。加入β-Lg后，负载EGCG的壳聚糖纳米颗粒（EGCG-CS NPs）的平均粒径从190 nm减小到157 nm，而包封效率（EE）从59.79％增加到76.29％。透射电子显微镜（TEM）的结果表明，所得纳米颗粒具有明显的核-壳结构。模拟胃肠道消化的结果表明，CS /β-LgNPs中EGCG的释放速率远低于CS-NPs。与免费的EGCG相比，DPPH和FRAP分析表明EGCG-CS NPs和EGCG-CS /β-LgNPs具有缓慢控制的抗氧化活性。同时，对细胞抗氧化活性（CAA）的研究表明，EGCG-CS NPs和EGCG-CS /β-LgNPs的EC50值分别降低了8.56％和18.35％。
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