Holographic detection of nanoparticles using acoustically actuated nanolenses.,Nature Communications

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Holographic detection of nanoparticles using acoustically actuated nanolenses.
Nature Communications ( IF 14.7 ) Pub Date : 2020-01-16 , DOI: 10.1038/s41467-019-13802-1
Aniruddha Ray _{1,

2,

3,

4,

5} , Muhammad Arslan Khalid ₆ , Andriejus Demčenko ₆ , Mustafa Daloglu _{1,

2,

3} , Derek Tseng _{1,

2,

3} , Julien Reboud ₆ , Jonathan M Cooper ₆ , Aydogan Ozcan _{1,

2,

3,

4}

Affiliation

The optical detection of nanoparticles, including viruses and bacteria, underpins many of the biological, physical and engineering sciences. However, due to their low inherent scattering, detection of these particles remains challenging, requiring complex instrumentation involving extensive sample preparation methods, especially when sensing is performed in liquid media. Here we present an easy-to-use, high-throughput, label-free and cost-effective method for detecting nanoparticles in low volumes of liquids (25 nL) on a disposable chip, using an acoustically actuated lens-free holographic system. By creating an ultrasonic standing wave in the liquid sample, placed on a low-cost glass chip, we cause deformations in a thin liquid layer (850 nm) containing the target nanoparticles (≥140 nm), resulting in the creation of localized lens-like liquid menisci. We also show that the same acoustic waves, used to create the nanolenses, can mitigate against non-specific, adventitious nanoparticle binding, without the need for complex surface chemistries acting as blocking agents.

中文翻译：

使用声驱动纳米透镜对纳米颗粒进行全息检测。

包括病毒和细菌在内的纳米颗粒的光学检测是许多生物、物理和工程科学的基础。然而，由于它们固有的散射较低，这些颗粒的检测仍然具有挑战性，需要复杂的仪器，涉及广泛的样品制备方法，特别是在液体介质中进行传感时。在这里，我们提出了一种易于使用、高通量、无标记且经济高效的方法，使用声驱动无透镜全息系统在一次性芯片上检测少量液体 (25 nL) 中的纳米颗粒。通过在放置在低成本玻璃芯片上的液体样品中产生超声波驻波，我们导致包含目标纳米粒子（≥140 nm）的薄液体层（850 nm）变形，从而产生局部透镜-像液体半月板。我们还表明，用于制造纳米透镜的相同声波可以减轻非特异性、外来纳米颗粒的结合，而不需要复杂的表面化学物质作为阻断剂。

更新日期：2020-01-16

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