Semiconducting polymers (SPs)-based dual photothermal therapy (PTT) obtained better therapeutic effect than single PTT due to its higher photothermal conversion efficiency. However, most dual PTT need to use two lasers for heat generation, which brings about inconvenience and limitation to the experimental operations. Herein, we report the development of “nanococktail” nanomaterials (DTPR) with 808 nm-activated image-guided dual photothermal properties for optimized cancer therapy.

Methods: In this work, we co-encapsulated AIEgens (TPA-BDTO, T) and SPs (PDPPP, P) by using maleimide terminated amphiphilic polymer (DSPE-PEG₂₀₀₀-Mal, D), then further conjugated the targeting ligands (RGD, R) through “click” reaction. Finally, such dual PTT nanococktail (termed as DTPR) was constructed.

Results: Once DTPR upon irradiation with 808 nm laser, near-infrared fluorescence from T could be partially converted into thermal energy through fluorescence resonance energy transfer (FRET) between T and P, coupling with the original heat energy generated by the photothermal agent P itself, thus resulting in image-guided dual PTT. The photothermal conversion efficiency of DTPR reached 60.3% (dual PTT), much higher as compared to its inherent photothermal effect of only 31.5% (single PTT), which was further proved by the more severe photothermal ablation in vitro and in vivo upon 808 nm laser irradiation.

Conclusion: Such smart “nanococktail” nanomaterials could be recognized as a promising photothermal nanotheranostics for image-guided cancer treatment.

基于半导体聚合物（SPs）的双重光热疗法（PTT）由于其更高的光热转换效率而获得了比单一PTT更好的治疗效果。然而，大多数双PTT需要使用两个激光器产生热量，这给实验操作带来不便和限制。在此，我们报告了具有 808 nm 激活图像引导双光热特性的“nanococktail”纳米材料 (DTPR) 的开发，用于优化癌症治疗。

方法：在这项工作中，我们使用马来酰亚胺封端的两亲性聚合物（DSPE-PEG ₂₀₀₀ -Mal ，D）将AIEgens（TPA-BDTO，T）和SP（PDPPP，P）共封装，然后进一步缀合靶向配体（RGD） ，R）通过“点击”反应。最后，构建了这种双 PTT 纳米混合物（称为 DTPR）。

结果：DTPR在808 nm激光照射下，T的近红外荧光可以通过T和P之间的荧光共振能量转移（FRET）部分转化为热能，与光热剂P本身产生的原始热能耦合，从而产生图像引导的双 PTT。DTPR的光热转换效率达到60.3%（双PTT），远高于其固有的光热效应仅为31.5%（单PTT），这进一步被808 nm下更严重的体外和体内光热消融所证明。激光照射。

结论：这种智能“纳米鸡尾酒”纳米材料可以被认为是一种有前景的用于图像引导癌症治疗的光热纳米治疗学。