Wide field fluorescence microscopy is the most commonly employed fluorescence imaging modality. However, a major drawback of wide field imaging is the very limited imaging depth in scattering samples. By experimentally varying the control of illumination, we found that the optimized illumination profile can lead to large contrast improvement for imaging at a depth beyond four scattering path lengths. At such imaging depth, we found that the achieved image signal-to-noise ratio can rival that of confocal measurement. As the employed illumination control is very simple, the method can be broadly applied to a wide variety of wide field fluorescence imaging systems.

中文翻译：

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通过简单的照明控制获得的对比度增益，用于散射样品的宽视野荧光成像。

宽视野荧光显微镜是最常用的荧光成像方法。但是，宽视场成像的主要缺点是散射样本中的成像深度非常有限。通过实验地改变照明的控制，我们发现优化的照明轮廓可以导致深度超过四个散射路径长度的成像的对比度大大提高。在这样的成像深度下，我们发现所获得的图像信噪比可以与共焦测量相媲美。由于所采用的照明控制非常简单，因此该方法可以广泛地应用于各种各样的宽视场荧光成像系统。