Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation.,PLOS Genetics

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Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation.
PLOS Genetics ( IF 4.0 ) Pub Date : 2020-01-15 , DOI: 10.1371/journal.pgen.1008546
Máté Pálfy ₁ , Gunnar Schulze ₂ , Eivind Valen _{2,

3} , Nadine L Vastenhouw ₁

Affiliation

In many organisms, early embryonic development is driven by maternally provided factors until the controlled onset of transcription during zygotic genome activation. The regulation of chromatin accessibility and its relationship to gene activity during this transition remain poorly understood. Here, we generated chromatin accessibility maps with ATAC-seq from genome activation until the onset of lineage specification. During this period, chromatin accessibility increases at regulatory elements. This increase is independent of RNA polymerase II-mediated transcription, with the exception of the hypertranscribed miR-430 locus. Instead, accessibility often precedes the transcription of associated genes. Loss of the maternal transcription factors Pou5f3, Sox19b, and Nanog, which are known to be required for zebrafish genome activation, results in decreased accessibility at regulatory elements. Importantly, the accessibility of regulatory regions, especially when established by Pou5f3, Sox19b and Nanog, is predictive for future transcription. Our results show that the maternally provided transcription factors Pou5f3, Sox19b, and Nanog open up chromatin and prime genes for activity during zygotic genome activation in zebrafish.

中文翻译：

Pou5f3、Sox19b 和 Nanog 建立的染色质可及性在斑马鱼基因组激活过程中启动基因的活性。

在许多生物体中，早期胚胎发育是由母体提供的因素驱动的，直到合子基因组激活期间转录的受控开始。在这一转变过程中，染色质可及性的调节及其与基因活性的关系仍然知之甚少。在这里，我们使用 ATAC-seq 生成了从基因组激活到谱系规范开始的染色质可及性图谱。在此期间，调节元件处的染色质可及性增加。这种增加与 RNA 聚合酶 II 介导的转录无关，但超转录的 miR-430 基因座除外。相反，可及性通常先于相关基因的转录。已知斑马鱼基因组激活所需的母体转录因子 Pou5f3、Sox19b 和 Nanog 的丢失会导致调节元件的可及性降低。重要的是，调节区域的可及性，尤其是由 Pou5f3、Sox19b 和 Nanog 建立的调节区域，可以预测未来的转录。我们的结果表明，母体提供的转录因子 Pou5f3、Sox19b 和 Nanog 在斑马鱼合子基因组激活过程中打开染色质和启动基因的活性。

更新日期：2020-02-18

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