Sandalwood oil has been widely used in perfumery industries and aromatherapy. Santalols are its major components. Herein, we attempted to construct santalol-producing yeasts. To alter flux from predominant triterpenoid/steroid biosynthesis to sesquiterpenoid production, expression of ERG9 (encoding yeast squalene synthase) was depressed by replacing its innate promotor with PHXT1 and fermenting the resulting strains in galactose-rich media. And the genes related to santalol biosynthesis were overexpressed under control of GAL promotors, which linked santalol biosynthesis to GAL regulatory system. GAL4 (a transcriptional activator of GAL promotors) and PGM2 (a yeast phosphoglucomutase) were overexpressed to overall promote this artificial santalol biosynthetic pathway and enhance galactose uptake. 1.3 g/L santalols and 1.2 g/L Z-α-santalol were achieved in the strain WL17 expressing SaSS (α-santalene synthase from Santalum album) and WL19 expressing SanSyn (α-santalene synthase from Clausena lansium) by fed-batch fermentation, respectively. This study constructed the microbial santalol-producing platform for the first time.

中文翻译：

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酵母中GAL调控系统控制下的檀香酚生物合成途径的重建。

檀香油已广泛用于香料行业和芳香疗法。桑塔洛尔是其主要成分。在本文中，我们试图构建生产檀香醇的酵母。为了改变从主要的三萜/类固醇生物合成到倍半萜的产生的通量，通过用PHXT1取代其先天启动子并在富含半乳糖的培养基中发酵所得菌株来抑制ERG9（编码酵母角鲨烯合酶）的表达。檀香醇生物合成相关基因在GAL启动子的控制下过表达，从而将檀香醇生物合成与GAL调控系统联系起来。GAL4（GAL启动子的转录激活剂）和PGM2（酵母磷酸葡糖突变酶）被过表达，以总体上促进这种人工檀香酚的生物合成途径并增强半乳糖的摄取。1.3 g / L檀香酚和1。通过分批分批发酵分别在表达SaSS的WL17菌株（来自Santalum Album的α-檀香烯合酶）和表达SanSyn的WL19菌株（来自Clausena lans的α-檀香烷合酶）中获得了2 g / LZ-α-檀香醇。该研究首次构建了微生物桑坦洛尔生产平台。