Angiosperm reproductive development is a complex event that includes floral organ development, male and female gametophyte formation and interaction between the male and female reproductive organs for successful fertilization. Previous studies have revealed the redundant function of ATP binding cassette subfamily G (ABCG) transporters ABCG1 and ABCG16 in pollen development, but whether they are involved in other reproductive processes is unknown. Here we show that ABCG1 and ABCG16 were not only expressed in anthers and stamen filaments but also enriched in pistil tissues, including the stigma, style, transmitting tract and ovule. We further demonstrated that pistil‐expressed ABCG1 and ABCG16 promoted rapid pollen tube growth through their effects on auxin distribution and auxin flow in the pistil. Moreover, disrupted auxin homeostasis in stamen filaments was associated with defective filament elongation. Our work reveals the key functions of ABCG1 and ABCG16 in reproductive development and provides clues for identifying ABCG1 and ABCG16 substrates in Arabidopsis.

中文翻译：

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ATP结合盒转运蛋白ABCG1和ABCG16通过拟南芥中的生长素信号传导影响生殖发育。

被子植物的生殖发育是一个复杂的事件，包括花器官发育，雄性和雌性配子体的形成以及雄性和雌性生殖器官之间的相互作用以成功受精。先前的研究揭示了ATP结合盒亚家族G（ABCG）转运蛋白ABCG1和ABCG16在花粉发育中的冗余功能，但尚不清楚它们是否参与其他生殖过程。在这里，我们显示ABCG1和ABCG16不仅在花药和雄蕊花丝中表达，而且在雌蕊组织中丰富，包括柱头，花柱，传播途径和胚珠。我们进一步证明了雌蕊表达的ABCG1和ABCG16通过影响雌蕊中的生长素分布和生长素流动，促进了花粉管的快速生长。此外，雄蕊花丝中生长素稳态的破坏与花丝伸长不良有关。我们的工作揭示了ABCG1和ABCG16在生殖发育中的关键功能，并为鉴定拟南芥中的ABCG1和ABCG16底物提供了线索。