A novel, simple, and sensitive method has been developed for simultaneous determination of 14 heterocyclic aromatic amines in meat product using solid-phase extraction combined with ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry. The analytes could be separated within 7 min and identified using their retention times and mass. The developed method was validated based on the linearity, limits of quantification, precision, and accuracy. The recovery ranged from 52.3 to 97.5% with an acceptable standard deviation, which is not higher than 6%. The limits of quantitation ranged from 0.03 to 0.17 µg/kg. The selectivity and sensitivity were satisfactory in multiple reaction monitoring mode. The method was applied to commercial meat products, and the results demonstrated that the novel method has potential for the analysis of the targets in food matrices. This is the first work reporting the simultaneous quantification of 14 heterocyclic aromatic amines by means of ultrahigh-performance supercritical fluid chromatography coupled to tandem quadrupole mass spectrometry.

中文翻译：

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固相萃取和超临界液相色谱-三重四极杆质谱联用测定肉制品中的14种杂环芳香胺。

已开发出一种新颖，简单而灵敏的方法，该方法可通过固相萃取结合超高效超临界流体色谱与串联四极杆质谱联用同时测定肉制品中的14种杂环芳香胺。可以在7分钟内分离出分析物，并使用其保留时间和质量进行鉴定。基于线性度，定量限，精密度和准确性对开发的方法进行了验证。回收率从52.3％到97.5％不等，可接受的标准偏差不超过6％。定量限为0.03至0.17 µg / kg。在多反应监测模式下，选择性和灵敏度令人满意。该方法已应用于商业肉制品，结果表明，该新方法具有分析食品基质中目标的潜力。这是第一篇报道通过串联四极杆质谱联用的超高性能超临界流体色谱仪同时定量14种杂环芳族胺的工作。