Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

中文翻译：

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流式细胞术分选后 T 细胞功能的评估显示 p38 MAPK 激活。

通过抗体、温度、匀浆、缓冲液成分和有丝分裂原进行流式细胞术细胞分选的分离和制备过程中的细胞改变是众所周知的。相比之下，对仪器或分选过程本身引起的细胞改变知之甚少。我们系统地评估了细胞对不同分拣机诱导的物理力的反应。总之，流式细胞仪细胞分选诱导力可以影响细胞信号级联，尤其是 MAPK p38。流式细胞术分选后人原代 T 细胞的与 p38 MAPK 通路相关的功能测定确实导致轻微的生理调节，但没有功能障碍。© 2020 作者。Cytometry Part A 由 Wiley Periodicals, Inc. 代表 International Society for Advancement of Cytometry 出版。