Abstract The aim of this study was to develop an effective sample preparation procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in smoked fatty products of animal origin (fish, cheese and sausage). Two different approaches were tested: classical QuEChERS and procedure with the use of Enhanced Matrix Removal (EMR)-Lipid material. Two techniques of extract preconcentration: under nitrogen stream and with the use of dispersive liquid-liquid microextraction (DLLME), were also taken into consideration. All samples were analysed using gas chromatography-mass spectrometry. The results showed the optimised sample preparation procedure was composed of three steps: 1) QuEChERS extraction, 2) clean-up by EMR-Lipid material and 3) extract preconcentration by DLLME. The obtained recovery rates within the range of 50–120% were received for all compounds with relative standard deviation (RSD) values lower than 16.7%. The proposed method is fast and effective and can be successfully applied for PAHs determination in difficult matrices such as heat-treated food of animal origin with high fat content. The research also discovered the significance of the quality of the laboratory disposables. Contaminants present in plastic consumables can be transferred to the sample extract contributing to its contamination and can also lead to failure of analytical equipment.

中文翻译：

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QuEChERS-EMR-Lipid-DLLME方法在动物源性熏制食品中多环芳烃测定中的应用

摘要 本研究的目的是开发一种有效的样品前处理程序，用于测定动物源性熏制脂肪产品（鱼、奶酪和香肠）中的多环芳烃 (PAH)。测试了两种不同的方法：经典 QuEChERS 和使用增强型基质去除 (EMR)-脂质材料的程序。还考虑了两种提取物预浓缩技术：在氮气流下和使用分散液液微萃取 (DLLME)。使用气相色谱-质谱法分析所有样品。结果表明，优化的样品制备程序由三个步骤组成：1) QuEChERS 提取，2) EMR-Lipid 材料净化，3) DLLME 提取物预浓缩。对于相对标准偏差 (RSD) 值低于 16.7% 的所有化合物，获得的回收率在 50-120% 范围内。所提出的方法快速有效，可成功应用于困难基质中多环芳烃的测定，例如脂肪含量高的动物源性热处理食品。该研究还发现了实验室一次性用品质量的重要性。塑料耗材中存在的污染物会转移到样品提取物中，导致其污染，也可能导致分析设备故障。所提出的方法快速有效，可成功应用于困难基质中多环芳烃的测定，例如脂肪含量高的动物源性热处理食品。该研究还发现了实验室一次性用品质量的重要性。塑料耗材中存在的污染物会转移到样品提取物中，导致其污染，也可能导致分析设备故障。所提出的方法快速有效，可成功应用于困难基质中多环芳烃的测定，例如脂肪含量高的动物源性热处理食品。该研究还发现了实验室一次性用品质量的重要性。塑料耗材中存在的污染物会转移到样品提取物中，导致其污染，也可能导致分析设备故障。