Extracellular matrix (ECM) contains a wide array of complex proteins, growth factors and cytokines that regulate cell behavior and tissue development. ECM harvested from non-homologous ECM sources still provide a structural support and biochemical cues to cells for effective tissue remodeling. The aim of this study is to evaluate the effect of non-tissue specific decellularized ECM from porcine dermis loaded with biphasic calcium phosphate powder (BCP) in bone regeneration. Thermosensitive ECM hydrogels with BCP powder exhibited a porous morphology with a suitable injectability and increased mechanical stability. In-vitro studies using MC3T3-E1 pre osteoblast cells showed that the injectable ECM hydrogels were biocompatible and supported the osteogenic differentiation. The bone regeneration capacity of the injectable ECM hydrogels was evaluated in-vivo by implanting in rat femoral head for 4 and 8 weeks. Micro-CT and histological staining results indicated that the injectable ECM hydrogels loaded with BCP powder showed higher and improved bone formation compared with the unfilled defect. Injectable ECM loaded with BCP powder is a good potential biomaterial for non-load bearing bone regeneration application.

细胞外基质（ECM）包含多种调节细胞行为和组织发育的复杂蛋白质，生长因子和细胞因子。从非同源ECM来源收获的ECM仍为细胞提供结构支持和生化线索，以进行有效的组织重塑。这项研究的目的是评估来自猪真皮的非组织特异性脱细胞ECM对双相磷酸钙粉末（BCP）的骨再生作用。具有BCP粉末的热敏ECM水凝胶表现出多孔形态，具有合适的可注射性和增强的机械稳定性。使用MC3T3-E1前成骨细胞的体外研究表明，可注射的ECM水凝胶具有生物相容性并支持成骨细胞分化。通过植入大鼠股骨头4周和8周，体内评估可注射的ECM水凝胶的骨再生能力。显微CT和组织学染色结果表明，与未填充的缺损相比，载有BCP粉末的可注射ECM水凝胶显示出更高的骨形成并改善了骨形成。载有BCP粉末的可注射ECM是用于非承重骨再生应用的良好潜在生物材料。