Abstract In order to avoid the interference of autofluorescence and improve tissue penetration capability of carbon quantum dots (CQDs) in biological imaging, the synthesis of long-wavelength emission CQDs was achieved in this work. Boron and nitrogen co-doped CQDs (BN-CQDs) were synthesized by one-step microwave assisted hydrothermal method with o-phenylenediamine as carbon and nitrogen source, boric acid as boron source. The BN-CQDs (QY = 13.79%) synthesized under the optimal conditions exhibit excitation independence, a large Stoke shift, satisfactory fluorescent stability, good biocompatibility and low toxicity, which can meet the basic requirements as cell imaging agent. Cell imaging measurements prove their increased intracellular accumulation with the extension of incubation time with HeLa cells, and clearly observable BN-CQDs imaging for cell morphology. Hence BN-CQDs can be used as a good cell imaging agent.

中文翻译：

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用于细胞成像的 BN 共掺杂黄色发光碳量子点的快速合成

摘要 为了避免自发荧光的干扰，提高碳量子点（CQDs）在生物成像中的组织穿透能力，本工作实现了长波长发射CQDs的合成。【摘要】：以邻苯二胺为碳源和氮源,硼酸为硼源,采用一步法微波辅助水热法合成了硼氮共掺杂碳量子点(BN-CQDs)。在最佳条件下合成的 BN-CQDs (QY = 13.79%) 表现出激发独立性、大斯托克位移、令人满意的荧光稳定性、良好的生物相容性和低毒性，可以满足作为细胞显像剂的基本要求。细胞成像测量证明，随着与 HeLa 细胞的孵育时间延长，它们的细胞内积累增加，和清晰可观察的 BN-CQDs 成像的细胞形态。因此，BN-CQDs 可以用作良好的细胞显像剂。