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Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis.
Current Biology ( IF 8.1 ) Pub Date : 2020-01-16 , DOI: 10.1016/j.cub.2019.11.078
Sacha Escamez 1 , Domenique André 1 , Bernadette Sztojka 1 , Benjamin Bollhöner 1 , Hardy Hall 1 , Béatrice Berthet 2 , Ute Voß 3 , Amnon Lers 4 , Alexis Maizel 2 , Magnus Andersson 5 , Malcolm Bennett 3 , Hannele Tuominen 1
Affiliation  

Plant organ growth is widely accepted to be determined by cell division and cell expansion, but, unlike that in animals, the contribution of cell elimination has rarely been recognized. We investigated this paradigm during Arabidopsis lateral root formation, when the lateral root primordia (LRP) must traverse three overlying cell layers within the parent root. A subset of LRP-overlying cells displayed the induction of marker genes for cell types undergoing developmental cell death, and their cell death was detected by electron, confocal, and light sheet microscopy techniques. LRP growth was delayed in cell-death-deficient mutants lacking the positive cell death regulator ORESARA1/ANAC092 (ORE1). LRP growth was restored in ore1-2 knockout plants by genetically inducing cell elimination in cells overlying the LRP or by physically killing LRP-overlying cells by ablation with optical tweezers. Our results support that, in addition to previously discovered mechanisms, cell elimination contributes to regulating lateral root emergence.

中文翻译:

覆盖侧根原基的细胞中的细胞死亡促进了拟南芥的器官生长。

植物器官的生长被广泛认为是由细胞分裂和细胞扩张决定的,但与动物不同,细胞消除的贡献很少被认可。我们在拟南芥侧根形成过程中研究了这种范例,当侧根原基 (LRP) 必须穿过亲根内的三个上覆细胞层时。LRP 上覆细胞的一个子集显示了对经历发育性细胞死亡的细胞类型的标记基因的诱导,并通过电子、共聚焦和光片显微镜技术检测到它们的细胞死亡。LRP 生长在缺乏阳性细胞死亡调节因子 ORESARA1/ANAC092 (ORE1) 的细胞死亡缺陷突变体中延迟。通过基因诱导 LRP 上覆的细胞中的细胞消除或通过用光镊消融物理杀死 LRP 上覆的细胞,可以在 ore1-2 敲除植物中恢复 LRP 生长。我们的结果支持,除了先前发现的机制外,细胞消除有助于调节侧根的出现。
更新日期:2020-01-16
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