The aim of this study was to characterize the proteins present in milk whey from buffaloes with and without subclinical mastitis using a proteomic approach to identify differentially expressed proteins as potential biomarkers for this disease. Whey from Murrah buffaloes with subclinical mastitis was compared with whey from healthy animals using liquid chromatography-tandem mass spectrometry. The annotated protein databases for Bubalus bubalis and Bos taurus were used in the analysis, and the gene annotations from the buffalo and bovine reference assemblies were also used. After integrating gene annotations from both buffaloes and bovines, a total of 1,033 proteins were identified, of which 156 were differentially expressed. Eighteen biological processes were annotated with Gene Ontology. Cathelicidin-3 was identified as a potential biomarker for subclinical mastitis. These results are important to the characterization of mastitis in the buffalo mammary gland and may aid in the development of tools for early diagnosis.

中文翻译：

---

简短交流：带有和不带有亚临床乳腺炎的乳牛的乳蛋白表达谱的表征。

这项研究的目的是使用蛋白质组学方法鉴定存在或不存在亚临床乳腺炎的水牛乳清中存在的蛋白质，以鉴定差异表达的蛋白质作为该疾病的潜在生物标记。使用液相色谱-串联质谱法比较了患有亚临床乳腺炎的Murrah水牛的乳清与健康动物的乳清。分析中使用了Bubalus bubalis和Bos taurus的注释蛋白质数据库，还使用了水牛和牛参考组件的基因注释。整合了来自水牛和牛的基因注释后，共鉴定出1033种蛋白质，其中156种差异表达。基因本体论注释了18个生物学过程。Cathelicidin-3被确定为亚临床乳腺炎的潜在生物标志物。这些结果对于表征水牛乳腺中的乳腺炎很重要，并且可能有助于早期诊断工具的开发。