Temperature-responsive films of poly(N-isopropylacrylamide) (PNIPAM) were facilely fabricated by one-step plasma-initiated polymerization and then functionalized by self-polymerization of dopamine on the surface. High retention of the monomer structure and temperature-responsive properties in PNIPAM films were confirmed. After plasma-initiated polymerization, PNIPAM films formed protrusions and ridges surfaces. Moreover, cross-linker of N,N′-methylenebisacrylamide with different dosage was introduced into the systems to effectively modulate the roughness of PNIPAM films and to supply better adhesive surface. Furthermore, in cell culture, satisfactory survival rate of the attached Hela cells was obtained on PNIPAM films, and the cell viability was improved further on PNIPAM/PDA films. The results indicated that such films might be applicable in medical treatment and tissue engineering, due to their adjusted adhesion ability and less toxicity to cells.

中文翻译：

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等离子体引发的 N-异丙基丙烯酰胺聚合并用多巴胺功能化以粘附于 Hela 细胞

聚（N-异丙基丙烯酰胺）（PNIPAM）的温度响应薄膜是通过一步等离子体引发的聚合轻松制造的，然后通过表面多巴胺的自聚合进行功能化。PNIPAM 薄膜中单体结构和温度响应特性的高保留得到证实。等离子引发聚合后，PNIPAM 薄膜形成突起和脊表面。此外，将不同用量的N,N'-亚甲基双丙烯酰胺交联剂引入体系中，有效调节PNIPAM薄膜的粗糙度并提供更好的粘合表面。此外，在细胞培养中，PNIPAM 膜上贴壁 Hela 细胞的存活率令人满意，PNIPAM/PDA 膜上的细胞活力进一步提高。