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Molecular interactions between Pluronic F127 and saponin in aqueous solution
Colloid and Polymer Science ( IF 2.2 ) Pub Date : 2020-01-16 , DOI: 10.1007/s00396-019-04552-z
Rosemaire S. S. de Oliveira , Nélida S. Marín Huachaca , Monique Lemos , Natália F. Santos , Eloi Feitosa , Luiz C. Salay

Nanotechnology is important for the development of new materials capable to improve the development of novel drug delivery systems. Such systems may result in more efficient therapeutic effects with reduced toxicity and extended time in the organism and increase in bioavailability, biocompatibility, and solubility of drugs. Here it was investigated the interaction between the triblock copolymer Pluronic F127 (F127) and a natural bioactive amphiphilic saponin (SAP), extracted from Glycyrrhiza glabra roots, aiming to form micelle-like F127-SAP complexes in a pH 7.4 HEPES buffered aqueous solution, with potential nanobiotechnological applications. Differential scanning calorimetry (DSC), dynamic light scattering (DLS), and dynamic electrophoretic light scattering (DESL) were the techniques used in this investigation, at varied SAP concentrations below and above CMC at fixed 500 μM F127. The SAP-F127 molecular interactions were verified by changes in the critical micellar temperature (CMT) of F127 obtained from the DSC thermograms, while DLS and DELS data showed, respectively, the formation larger structures of the complexes relative to the single Pluronic micelles and zeta potential (ζ) values ranging from ca − 6.5 to − 9 mV, on varying the SAP concentration. Combined, these results indicate the formation of micelle-like complexes, with structure dependent on the SAP concentration, as highly promising agents in nanobiotechnology applications.

中文翻译:

Pluronic F127与皂苷在水溶液中的分子相互作用

纳米技术对于开发能够改进新型药物递送系统开发的新材料非常重要。此类系统可产生更有效的治疗效果,同时降低毒性并延长生物体内的时间,并增加药物的生物利用度、生物相容性和溶解度。在这里,研究了三嵌段共聚物 Pluronic F127 (F127) 和从光果甘草根中提取的天然生物活性两亲皂苷 (SAP) 之间的相互作用,旨在在 pH 7.4 的 HEPES 缓冲水溶液中形成胶束状 F127-SAP 复合物,具有潜在的纳米生物技术应用。差示扫描量热法 (DSC)、动态光散射 (DLS) 和动态电泳光散射 (DESL) 是本研究中使用的技术,在低于和高于 CMC 的不同 SAP 浓度下,固定 500 μM F127。SAP-F127 分子相互作用通过从 DSC 热谱图中获得的 F127 临界胶束温度 (CMT) 的变化得到验证,而 DLS 和 DELS 数据分别显示,相对于单个 Pluronic 胶束和 zeta,复合物的形成更大的结构在改变 SAP 浓度时,电位 (ζ) 值范围从 ca - 6.5 到 - 9 mV。综合起来,这些结果表明胶束状复合物的形成,其结构取决于 SAP 浓度,是纳米生物技术应用中非常有前途的试剂。分别地,相对于单个 Pluronic 胶束和 zeta 电位 (ζ) 值范围从 ca - 6.5 到 - 9 mV,随着 SAP 浓度的变化,复合物的形成更大的结构。综合起来,这些结果表明胶束状复合物的形成,其结构取决于 SAP 浓度,是纳米生物技术应用中非常有前途的试剂。分别地,相对于单个 Pluronic 胶束和 zeta 电位 (ζ) 值范围从 ca - 6.5 到 - 9 mV,随着 SAP 浓度的变化,复合物的形成更大的结构。综合起来,这些结果表明胶束状复合物的形成,其结构取决于 SAP 浓度,是纳米生物技术应用中非常有前途的试剂。
更新日期:2020-01-16
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