Extensive use of organophosphorus pesticides (OPs) in crop protection has aroused worldwidely great concern about safety and the detection of OPs is of great significance to food safety and human health. In this work, peptides attached with tetraphenylethylene (TPE) molecule were synthesized to from an aggregation-induced emission fluorescent probe (TPE-Peptide) for the determination of OPs. The working mechanism was as follows: in presence of OPs, OPs would react with active site serine in the peptide sequence via covalent bond and adducts were formed between OPs and the peptides; once formed, the adducts accelerated the aggregation of peptides, thus inducing strong emission of TPE-Peptide probe. So the adducts formation and the enhanced emission of the TPE-Peptide probe were the key factors for the OPs' sensing. Herein, the adducts formed between OPs and TPE-Peptide probe, the aggregated peptide fibrils were characterized by fluorescence, mass spectrometry, transmission electron microscopy, dynamic light scattering, atomic force microscopy, circular dichroism spectra and confocal fluorescence microscopy etc. This TPE-Peptide probe displayed highly sensitive fluorescence response where OPs' concentrations ranged from 1 to 100 μM with the limit of detection 0.6 μM and also showed selectivity.

中文翻译：

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用于有机磷农药检测的荧光肽探针。

有机磷农药（OPs）在作物保护中的广泛使用引起了全世界对安全性的高度关注，而OPs的检测对食品安全和人类健康具有重要意义。在这项工作中，从聚集诱导发射荧光探针（TPE-肽）合成了附着有四苯基乙烯（TPE）分子的肽，用于测定OP。其工作机制如下：在存在OP的情况下，OP会通过共价键与肽序列中的活性位点丝氨酸反应，并在OP与肽之间形成加合物。一旦形成，加合物就加速了肽的聚集，从而诱导了TPE-肽探针的强发射。因此，TPE-肽探针的加合物形成和发射增强是OP感测的关键因素。在这里