Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor signaling in immune-competent human cell lines,Nanoscale Advances

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Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor signaling in immune-competent human cell lines
Nanoscale Advances ( IF 4.6 ) Pub Date : 2020/01/15 , DOI: 10.1039/c9na00721k
Anda R Gliga _{1,

2} , Jessica De Loma ₁ , Sebastiano Di Bucchianico ₁ , Sara Skoglund ₃ , Sandeep Keshavan ₂ , Inger Odnevall Wallinder ₃ , Hanna L Karlsson ₁ , Bengt Fadeel ₂

Affiliation

Silver (Ag) nanoparticles are commonly used in consumer products due to their antimicrobial properties. Here we studied the impact of Ag nanoparticles on immune responses by using cell lines of monocyte/macrophage and lung epithelial cell origin, respectively. Short-term experiments (24 h) showed that Ag nanoparticles reduced the lipopolysaccharide (LPS)-induced secretion of pro-inflammatory cytokines in THP-1 cells under serum-free conditions. ICP-MS analysis revealed that cellular uptake of Ag was higher under these conditions. Long-term exposure (up to 6 weeks) of BEAS-2B cells to Ag nanoparticles also suppressed pro-inflammatory cytokine production following a brief challenge with LPS. Experiments using reporter cells revealed that Ag nanoparticles as well as AgNO₃ inhibited LPS-triggered Toll-like receptor (TLR) signaling. Furthermore, RNA-sequencing of BEAS-2B cells indicated that Ag nanoparticles affected TLR signaling pathways. In conclusion, Ag nanoparticles reduced the secretion of pro-inflammatory cytokines in response to LPS, likely as a result of the release of silver ions leading to an interference with TLR signaling. This could have implications for the use of Ag nanoparticles as antibacterial agents. Further in vivo studies are warranted to study this.

中文翻译：

银纳米粒子调节免疫活性人类细胞系中脂多糖触发的 Toll 样受体信号传导

银 (Ag) 纳米粒子因其抗菌特性而常用于消费品中。在这里，我们分别使用单核细胞/巨噬细胞和肺上皮细胞来源的细胞系研究了 Ag 纳米颗粒对免疫反应的影响。短期实验（24 小时）表明，在无血清条件下，银纳米粒子可减少 THP-1 细胞中脂多糖 (LPS) 诱导的促炎细胞因子分泌。ICP-MS 分析表明，在这些条件下，细胞对 Ag 的吸收更高。在短暂的 LPS 挑战后，BEAS-2B 细胞长期暴露于 Ag 纳米颗粒（长达 6 周）也抑制了促炎细胞因子的产生。使用报告细胞的实验表明，Ag 纳米颗粒和 AgNO ₃抑制 LPS 触发的 Toll 样受体 (TLR) 信号传导。此外，BEAS-2B 细胞的 RNA 测序表明 Ag 纳米颗粒影响 TLR 信号通路。总之，Ag 纳米颗粒减少了响应 LPS 的促炎细胞因子的分泌，这可能是由于银离子的释放导致 TLR 信号传导受到干扰。这可能对使用银纳米粒子作为抗菌剂有影响。有必要进行进一步的体内研究来研究这一点。

更新日期：2020-02-19

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