Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering,RSC Advances

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Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering
RSC Advances ( IF 3.9 ) Pub Date : 2020-1-15 , DOI: 10.1039/c9ra09531d
Luyu Wang ₁ , Jinrui Li ₁ , Dan Zhang ₁ , Shanshan Ma ₁ , Junni Zhang ₁ , Feng Gao ₁ , Fangxia Guan ₁ , Minghao Yao ₁

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Recently, in situ formed injectable hydrogels have shown great potential in biomedical applications as therapeutic implants or carriers in tissue repair and regeneration. They can seal or fill the damaged tissue to function as cell/drug delivery vehicle perfectly through a minimally invasive surgical procedure. In this study, hyaluronic acid (HA) is functionalized with tyramine to produce an injectable hydrogel dual-enzymatically crosslinked by horseradish peroxidase (HRP) and galactose oxidase (GalOX). This new tyramine-modified HA (HT) hydrogel exhibited good injectability, favorable cytocompatibility to mice bone marrow mesenchymal stem cells (BMSCs), and low inflammatory response verified by cytotoxicity assay in vitro and an in situ subcutaneous injection study in vivo. In addition, the gelation time, swelling behavior, and degradation rate of the HT hydrogel could be adjusted through varying the concentrations of HT and GalOX in a certain range. These encouraging results suggest that such biocompatible HT hydrogels might have potential application in three-dimensional stem cell culture and tissue engineering.

中文翻译：

双酶交联可注射透明质酸水凝胶在组织工程中的潜在应用

最近，原位形成的可注射水凝胶在生物医学应用中显示出巨大的潜力，作为组织修复和再生的治疗植入物或载体。它们可以通过微创外科手术密封或填充受损组织，从而完美地充当细胞/药物输送载体。在这项研究中，透明质酸 (HA) 与酪胺功能化，以产生可注射的水凝胶，通过辣根过氧化物酶 (HRP) 和半乳糖氧化酶 (GalOX) 进行双酶交联。这种新型酪胺修饰的 HA (HT) 水凝胶具有良好的可注射性，对小鼠骨髓间充质干细胞 (BMSCs) 具有良好的细胞相容性，并通过体外和原位细胞毒性试验验证了低炎症反应体内皮下注射研究。此外，HT水凝胶的凝胶时间、溶胀行为和降解速率可以通过在一定范围内改变HT和GalOX的浓度来调节。这些令人鼓舞的结果表明，这种生物相容的 HT 水凝胶可能在三维干细胞培养和组织工程中具有潜在应用。

更新日期：2020-01-15

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