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Investigation of the early healing response to dicationic imidazolium-based ionic liquids: a biocompatible coating for titanium implants.
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-01-24 , DOI: 10.1021/acsbiomaterials.9b01884 Sutton E Wheelis 1 , Claudia C Biguetti 2, 3 , Shruti Natarajan 4 , Lidia Guida 1 , Brian Hedden 1 , Gustavo P Garlet 3 , Danieli C Rodrigues 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-01-24 , DOI: 10.1021/acsbiomaterials.9b01884 Sutton E Wheelis 1 , Claudia C Biguetti 2, 3 , Shruti Natarajan 4 , Lidia Guida 1 , Brian Hedden 1 , Gustavo P Garlet 3 , Danieli C Rodrigues 1
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Dicationic imidazolum-based ionic liquids with amino acid anions (IonL) have been proposed as a multifunctional coating for titanium dental implants, as their properties have been shown to address multiple early complicating factors while maintaining host cell compatibility. This study aims to evaluate effects of this coating on host response in the absence of complicating oral factors during the early healing period using a subcutaneous implantation model in the rat. IonLs with the best cytocompatibility and antimicrobial properties (IonL-Phe, IonL-Met) were chosen as coatings. Three different doses were applied to cpTi disks and subcutaneously implanted into 36 male Lewis rats. Rats received two implants: one coated implant on one side and an uncoated implant on the contralateral sides (n = 3 per formulation, per dose). Peri-implant tissue was evaluated 2 and 14 days after implantation with H&E staining and IHC markers associated with macrophage polarization as well as molecular analysis (qPCR) for inflammatory and healing markers. H&E stains revealed the presence of the coating, blood clots, and inflammatory infiltrate at 2 days around all implants. At 14 days, inflammation had receded with more developed connective tissue with fibroblasts, blood vessels in certain doses of coated and uncoated samples with no foreign body giant cells. This study demonstrated that IonL at the appropriate concentration does not significantly interfere with healing and Ti foreign body response. Results regarding optimal dose and formulation from this study will be applied in future studies using an oral osseointegration model.
中文翻译：
对基于咪唑鎓的离子型离子液体的早期愈合反应的研究：钛植入物的生物相容性涂层。
含氨基酸阴离子的二阳离子咪唑基离子液体（IonL）已被提议作为钛牙科植入物的多功能涂料，因为它们的性能已显示出可解决多种早期复杂因素，同时又能保持宿主细胞相容性。这项研究的目的是在大鼠的早期愈合阶段，在没有复杂口腔因素的情况下，使用皮下植入模型评估这种涂层对宿主反应的影响。选择具有最佳细胞相容性和抗菌特性的IonL（IonL-Phe，IonL-Met）作为涂层。将三种不同的剂量应用于cpTi盘，并皮下植入36只雄性Lewis大鼠。大鼠接受了两种植入物：一侧是带涂层的植入物，对侧是未涂层的植入物（n=每个制剂每剂量3个）。植入后2天和14天用H＆E染色和与巨噬细胞极化相关的IHC标记物以及炎症和愈合标记物的分子分析（qPCR）对植入物周围组织进行评估。H＆E染色显示所有植入物在第2天存在涂层，血块和炎性浸润。在第14天，发炎的结缔组织，成纤维细胞，血管在一定剂量的包被和未包被样品中均已消退，没有异物巨细胞。这项研究表明，适当浓度的IonL不会显着干扰愈合和Ti异物反应。该研究中有关最佳剂量和配方的结果将在未来的研究中使用口服骨整合模型。
更新日期：2020-01-26
中文翻译：
对基于咪唑鎓的离子型离子液体的早期愈合反应的研究：钛植入物的生物相容性涂层。
含氨基酸阴离子的二阳离子咪唑基离子液体（IonL）已被提议作为钛牙科植入物的多功能涂料，因为它们的性能已显示出可解决多种早期复杂因素，同时又能保持宿主细胞相容性。这项研究的目的是在大鼠的早期愈合阶段，在没有复杂口腔因素的情况下，使用皮下植入模型评估这种涂层对宿主反应的影响。选择具有最佳细胞相容性和抗菌特性的IonL（IonL-Phe，IonL-Met）作为涂层。将三种不同的剂量应用于cpTi盘，并皮下植入36只雄性Lewis大鼠。大鼠接受了两种植入物：一侧是带涂层的植入物，对侧是未涂层的植入物（n=每个制剂每剂量3个）。植入后2天和14天用H＆E染色和与巨噬细胞极化相关的IHC标记物以及炎症和愈合标记物的分子分析（qPCR）对植入物周围组织进行评估。H＆E染色显示所有植入物在第2天存在涂层，血块和炎性浸润。在第14天，发炎的结缔组织，成纤维细胞，血管在一定剂量的包被和未包被样品中均已消退，没有异物巨细胞。这项研究表明，适当浓度的IonL不会显着干扰愈合和Ti异物反应。该研究中有关最佳剂量和配方的结果将在未来的研究中使用口服骨整合模型。
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