Decreases in estrogen levels due to menopause or ovariectomy may disrupt cerebellar motor functions. This study aimed at investigating the effects of Moderate Intensity Intermittent Exercise (MIEx) on the cerebellum of ovariectomized rats by analyzing neurotrophic and neuroprotective markers, as well as cerebellar motor functions. Thirty-two female Sprague Dawley rats were divided into four groups, i.e. Sham and ovariectomy (Ovx) of non-MIEx (NMIEx) groups, and Sham and Ovx with MIEx groups. MIEx was performed 5 days a week on treadmill for 6 weeks. Motor functions were assessed using rotarod, footprint, open field, and wire hanging tests. Real-time polymerase chain reaction was performed to determine messenger RNA (mRNA) expressions of Pgc-1α, BDNF, synaptophysin, Bcl-2, and Bax. Unbiased stereology was used to estimate the total number of cerebellar Purkinje cells. The Ovx MIEx group had higher Pgc-1α and Bcl-2 mRNA expressions, and number of Purkinje cells, but lower Bax mRNA expression than the Ovx NMIEx group. All motor functions of MIEx groups were better than the Sham and Ovx groups without MIEx. Motor functions on rotarod task, OFT, and FPT correlated significantly with the mRNAs expression of Bcl-2, Bax, BDNF, synaptophysin, Pgc-1α, and the number of cerebellar Purkinje cells in ovariectomized rats. MIEx improves cerebellar neurotrophic and neuroprotective markers, as well as motor functions of ovariectomized rats.

中文翻译：

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中等强度的间歇运动可上调去卵巢大鼠小脑的神经营养和神经保护基因表达，并抑制Purkinje细胞丢失。

由于绝经或卵巢切除导致雌激素水平降低可能会破坏小脑运动功能。这项研究旨在通过分析神经营养和神经保护标志物以及小脑运动功能，研究中度间歇间歇运动（MIEx）对去卵巢大鼠小脑的影响。将32只雌性Sprague Dawley大鼠分为四组，即非MIEx（NMIEx）组的假手术和卵巢切除术（Ovx），以及MIEx组的Sham和Ovx。MIEx每周5天在跑步机上进行6周。使用旋转脚架，脚印，开阔野和电线悬挂测试评估了运动功能。进行实时聚合酶链反应以确定Pgc-1α，BDNF，突触素，Bcl-2和Bax的信使RNA（mRNA）表达。使用无偏立体学来估计小脑浦肯野细胞的总数。与Ovx NMIEx组相比，Ovx MIEx组具有更高的Pgc-1α和Bcl-2 mRNA表达以及Purkinje细胞数量，但Bax mRNA表达却较低。MIEx组的所有运动功能均优于没有MIEx的Sham和Ovx组。在卵巢切除的大鼠中，轮状任务，OFT和FPT上的运动功能与Bcl-2，Bax，BDNF，突触素，Pgc-1α和小脑Purkinje细胞的mRNA的表达显着相关。MIEx可改善小脑神经营养和神经保护标记，以及去卵巢大鼠的运动功能。MIEx组的所有运动功能均优于没有MIEx的Sham和Ovx组。在卵巢切除的大鼠中，轮状任务，OFT和FPT上的运动功能与Bcl-2，Bax，BDNF，突触素，Pgc-1α和小脑Purkinje细胞的mRNA的表达显着相关。MIEx可改善小脑神经营养和神经保护标记，以及去卵巢大鼠的运动功能。MIEx组的所有运动功能均优于没有MIEx的Sham和Ovx组。在切除卵巢的大鼠中，轮状任务，OFT和FPT上的运动功能与Bcl-2，Bax，BDNF，突触素，Pgc-1α和小脑浦肯野细胞的mRNA表达密切相关。MIEx可改善小脑神经营养和神经保护标记，以及去卵巢大鼠的运动功能。