A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode,Biotechnology and Applied Biochemistry

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A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-01-15 , DOI: 10.1002/bab.1886
Onur Can Bodur ₁ , Saliha Dinç ₂ , Mustafa Özmen ₃ , Fatma Arslan ₁

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Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot‐(3‐Aminopropyl) triethoxysilane (CDs‐APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross‐linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H₂O₂ at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs‐APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.

中文翻译：

使用碳点修饰的碳糊电极对神经递质乙酰胆碱进行灵敏的安培检测

乙酰胆碱是一种神经递质，位于内脏运动系统淋巴结和中枢神经系统各个部位的神经和肌肉相交处。脑中乙酰胆碱的减少与阿尔茨海默氏病有关。这就是为什么它是这种疾病的重要因素。在这项研究中，使用碳纳米点-（3-氨基丙基）三乙氧基硅烷（CDs-APTES）修饰的碳糊电极制备了具有乙酰胆碱酯酶和胆碱氧化酶的双酶生物传感器系统，用于测定乙酰胆碱的量。通过与戊二醛交联将乙酰胆碱酯酶和胆碱氧化酶固定在修饰的碳糊电极上。乙酰胆碱的测定是通过酶促产生的H的氧化来进行的相对于Ag / AgCl，在0.4 V下为₂ O ₂。研究了温度，pH和底物浓度对制备的生物传感器的乙酰胆碱响应的影响。此外，还研究了最佳CDs-APTES量，生物传感器的线性工作范围以及干扰效应。

更新日期：2020-01-15

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