There is limited variability within rapeseed germplasm in Morocco. Induced mutation was recently used to generate novel genetic variability and develop mutant lines combining desirable traits. In this context, nine promising advanced rapeseed M₂ mutant lines and the wild‐type variety “INRA‐CZH2” were evaluated for their seed oil content, fatty acid composition, total phenolic content (TPC), and free‐radical scavenging activity (FRSA) by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and 3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) methods. The results showed significant variability among all mutants in seed oil content (38.14–42.04%) and fatty acids (SAFA = 5.49–10.99%, MUFA = 50.33–71.62%, PUFA = 22.89–8.68%). The mutant H2M‐5 exhibited the highest fraction of MUFA and the lowest proportion of SAFA and PUFA, while the mutant H2M‐4 showed the highest SAFA and PUFA amounts and the lowest MUFA level. TPC varied from 2.16 to 4.35 mg GAE/100 g. The highest amount was found in the mutant H2M‐1, which is about twice that of other mutants and the wild‐type variety. FRSA differed significantly among the samples, and the variations observed for DPPH and ABTS methods were 40.5–59.28% and 40.31–59.86%, respectively. FRSA was positively correlated to TPC in the sampled oils (r = 0.801 and 0.802, P < 0.01). This is the first report emphasizing the biochemical potential of rapeseed varieties and novel mutants in Morocco. H2M‐1, H2M‐4, and H2M‐5 were proposed for the Rapeseed National Breeding Program, as they showed higher levels in some biochemical traits of interest.

中文翻译：

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油菜突变体品系中脂肪酸组成，总酚类和抗氧化能力的比较研究

摩洛哥的油菜籽种质变异有限。诱导突变最近被用于产生新的遗传变异性，并发展结合了所需性状的突变系。在这种情况下，九种有前途的高级油菜籽M ₂通过2,2-二苯-1-基对突变株和野生型“ INRA-CZH2”的种子油含量，脂肪酸组成，总酚含量（TPC）和自由基清除活性（FRSA）进行了评估。苦瓜肼（DPPH）和3-乙基苯并噻唑啉-6-磺酸（ABTS）方法。结果显示，所有突变体的种子油含量（38.14–42.04％）和脂肪酸（SAFA = 5.49–10.99％，MUFA = 50.33–71.62％，PUFA = 22.89–8.68％）之间存在显着差异。H2M-5突变体的MUFA含量最高，SAFA和PUFA的比例最低，而H2M-4突变体的SAFA和PUFA量最高，MUFA含量最低。TPC从2.16到4.35 mg GAE / 100 g不等。在突变体H2M-1中发现的含量最高，约为其他突变体和野生型品种的两倍。样品之间的FRSA差异显着，DPPH和ABTS方法观察到的差异分别为40.5-59.28％和40.31-59.86％。样品油中FRSA与TPC正相关（r = 0.801和0.802，P <0.01）。这是第一份强调摩洛哥油菜籽品种和新型突变体生化潜力的报告。H2M-1，H2M-4和H2M-5被建议用于油菜籽国家育种计划，因为它们在某些感兴趣的生化特征上显示出较高的水平。