Glycerol is a byproduct of biodiesel production and is generated in large amounts, which has resulted in an increased interest in its valorization. In addition to its use as an energy source directly, the chemical modification of glycerol may result in value-added derivatives. Herein, acid phosphatases employed in the synthetic mode were evaluated for the enzymatic phosphorylation of glycerol. Nonspecific acid phosphatases could tolerate glycerol concentrations up to 80 wt % and pyrophosphate concentrations up to 20 wt % and led to product titers up to 167 g L-1 in a kinetic approach. In the complementary thermodynamic approach, phytases were able to condense glycerol and inorganic monophosphate directly. This unexpected behavior enabled the simple and cost-effective production of rac-glycerol-1-phosphate from crude glycerol obtained from a biodiesel plant. A preparative-scale synthesis on a 100 mL-scale resulted in the production of 16.6 g of rac-glycerol-1-phosphate with a reasonable purity (≈75 %).

中文翻译：

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使用酸性磷酸酶和磷酸盐从粗甘油制备规模规模的酶法合成rac-甘油-1-磷酸。

甘油是生物柴油生产的副产物，并大量产生，这引起了对其增值的兴趣增加。除了直接用作能源外，甘油的化学改性还可能产生增值的衍生物。在此，对合成模式中使用的酸性磷酸酶进行了甘油的酶促磷酸化的评价。非特异性酸性磷酸酶可以耐受高达80 wt％的甘油浓度和高达20 wt％的焦磷酸盐浓度，并通过动力学方法导致高达167 g L-1的产品滴定度。在互补热力学方法中，肌醇六磷酸酶能够直接冷凝甘油和无机单磷酸酯。这种出乎意料的行为使从生物柴油工厂获得的粗制甘油能够简单，经济高效地生产rac-甘油-1-磷酸。在100 mL规模上进行制备规模的合成后，生成了16.6 g rac-甘油1-磷酸酯，纯度适中（≈75％）。