Plasmonic Biosensor Controlled by DNAzyme for On-Site Genetic Detection of Pathogens.,Biotechnology Journal

当前位置： X-MOL 学术 › Biotechnol. J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Plasmonic Biosensor Controlled by DNAzyme for On-Site Genetic Detection of Pathogens.
Biotechnology Journal ( IF 3.2 ) Pub Date : 2020-03-04 , DOI: 10.1002/biot.201900329
Woogi Lee ₁ , Byeong Hee Hwang _{1,

2}

Affiliation

On-site predetection of pathogens could significantly decrease of a disease outbreak or national loss in most of the countries. However, conventional detection techniques are limited in use for on-site detection due to the necessity of specialized skill or equipment. Therefore, it is necessary to develop a new technique that can predetect pathogens in the field without special skills or equipment. Here, a DNAzyme strategy to control a plasmonic biosensor for rapid and simple visual detection of Salmonella choleraesuis is adopted. Multicomponent DNAzyme formed by target addition can cleave the linker effectively at 50 °C. Linker cleavage induces dispersion of two DNA-immobilized gold nanoparticles and color change. Under optimized assay conditions, the target could be detected via visual discrimination sensitively and specifically. Moreover, the biosensor shows the possibility of practical use with contaminants and a 16S rRNA real target. As a result, the proposed plasmonic biosensor can visually detect S. choleraesuis without unstable enzymes, a specialized technique, or equipment. Therefore, these advantages could allow that this biosensor would be used for on-site predetection to lower the risk of transmission of infectious diseases.

中文翻译：

DNAzyme控制的等离子生物传感器用于病原体的现场遗传检测。

在大多数国家中，病原体的现场预先检测可以显着减少疾病暴发或国家损失。但是，由于需要专门的技术或设备，传统的检测技术在现场检测中受到限制。因此，有必要开发一种无需特殊技能或设备即可预先检测病原体的新技术。在这里，采用了一种控制等离子生物传感器的DNAzyme策略，以便快速，简单地目测霍乱沙门氏菌。通过靶标添加形成的多组分DNA酶可在50°C下有效裂解接头。接头裂解引起两个固定DNA的金纳米颗粒的分散和颜色变化。在优化的测定条件下，可以通过视觉辨认灵敏而特异性地检测目标。此外，该生物传感器显示了与污染物和16S rRNA真正靶标实际应用的可能性。结果，提出的等离子体生物传感器可以在没有不稳定酶，专用技术或设备的情况下视觉地检测霍乱链球菌。因此，这些优点可以使该生物传感器用于现场预检测，以降低传染病传播的风险。

更新日期：2020-03-04

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11