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Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-17 , DOI: 10.1111/jipb.12907 Xiaoxiao Dong 1 , Sujuan Duan 1, 2 , Hong-Bin Wang 1 , Hong-Lei Jin 2
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-17 , DOI: 10.1111/jipb.12907 Xiaoxiao Dong 1 , Sujuan Duan 1, 2 , Hong-Bin Wang 1 , Hong-Lei Jin 2
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The balance between cellular carbon (C) and nitrogen (N) must be tightly coordinated to sustain optimal growth and development in plants. In chloroplasts, photosynthesis converts inorganic C to organic C, which is important for maintenance of C content in plant cells. However, little is known about the role of chloroplasts in C/N balance. Here, we identified a nuclear‐encoded protein LOW PHOTOSYNTHETIC EFFICIENCY2 (LPE2) that it is required for photosynthesis and C/N balance in Arabidopsis. LPE2 is specifically localized in the chloroplast. Both loss‐of‐function mutants, lpe2‐1 and lpe2‐2, showed lower photosynthetic activity, characterized by slower electron transport and lower PSII quantum yield than the wild type. Notably, LPE2 is predicted to encode the plastid ribosomal protein S21 (RPS21). Deficiency of LPE2 significantly perturbed the thylakoid membrane composition and plastid protein accumulation, although the transcription of plastid genes is not affected obviously. More interestingly, transcriptome analysis indicated that the loss of LPE2 altered the expression of C and N response related genes in nucleus, which is confirmed by quantitative real‐time‐polymerase chain reaction. Moreover, deficiency of LPE2 suppressed the response of C/N balance in physiological level. Taken together, our findings suggest that LPE2 plays dual roles in photosynthesis and the response to C/N balance.
中文翻译：
拟南芥中质体核糖体蛋白LPE2参与光合作用和对C / N平衡的响应。
细胞碳（C）和氮（N）之间的平衡必须紧密协调，以维持植物的最佳生长发育。在叶绿体中，光合作用将无机C转换为有机C，这对于维持植物细胞中C的含量很重要。然而，人们对叶绿体在C / N平衡中的作用知之甚少。在这里，我们确定了拟南芥中光合作用和C / N平衡所需的核编码蛋白低光合效率（LPE2）。LPE2专门位于叶绿体中。功能缺失的突变体lpe2-1和lpe2-2均显示出较低的光合作用活性，其特征是电子传递较野生型慢，PSII量子产率较低。值得注意的是，LPE2预计其可编码质体核糖体蛋白S21（RPS21）。LPE2缺乏明显扰动类囊体膜组成和质体蛋白积累，虽然质体基因的转录没有受到明显影响。更有趣的是，转录组分析表明LPE2的丢失改变了细胞核中C和N反应相关基因的表达，这通过定量实时聚合酶链反应得以证实。此外，LPE2的缺乏在生理水平上抑制了C / N平衡的响应。综上所述，我们的发现表明LPE2在光合作用和C / N平衡响应中起着双重作用。
更新日期：2020-03-17
中文翻译：
拟南芥中质体核糖体蛋白LPE2参与光合作用和对C / N平衡的响应。
细胞碳（C）和氮（N）之间的平衡必须紧密协调，以维持植物的最佳生长发育。在叶绿体中，光合作用将无机C转换为有机C，这对于维持植物细胞中C的含量很重要。然而，人们对叶绿体在C / N平衡中的作用知之甚少。在这里，我们确定了拟南芥中光合作用和C / N平衡所需的核编码蛋白低光合效率（LPE2）。LPE2专门位于叶绿体中。功能缺失的突变体lpe2-1和lpe2-2均显示出较低的光合作用活性，其特征是电子传递较野生型慢，PSII量子产率较低。值得注意的是，LPE2预计其可编码质体核糖体蛋白S21（RPS21）。LPE2缺乏明显扰动类囊体膜组成和质体蛋白积累，虽然质体基因的转录没有受到明显影响。更有趣的是，转录组分析表明LPE2的丢失改变了细胞核中C和N反应相关基因的表达，这通过定量实时聚合酶链反应得以证实。此外，LPE2的缺乏在生理水平上抑制了C / N平衡的响应。综上所述，我们的发现表明LPE2在光合作用和C / N平衡响应中起着双重作用。
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