当前位置: X-MOL 学术Nat. Commun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Acquisition, transmission and strain diversity of human gut-colonizing crAss-like phages.
Nature Communications ( IF 14.7 ) Pub Date : 2020-01-15 , DOI: 10.1038/s41467-019-14103-3
Benjamin A Siranosian 1 , Fiona B Tamburini 1 , Gavin Sherlock 1 , Ami S Bhatt 1, 2
Affiliation  

CrAss-like phages are double-stranded DNA viruses that are prevalent in human gut microbiomes. Here, we analyze gut metagenomic data from mother-infant pairs and patients undergoing fecal microbiota transplantation to evaluate the patterns of acquisition, transmission and strain diversity of crAss-like phages. We find that crAss-like phages are rarely detected at birth but are increasingly prevalent in the infant microbiome after one month of life. We observe nearly identical genomes in 50% of cases where the same crAss-like clade is detected in both the mother and the infant, suggesting vertical transmission. In cases of putative transmission of prototypical crAssphage (p-crAssphage), we find that a subset of strains present in the mother are detected in the infant, and that strain diversity in infants increases with time. Putative tail fiber proteins are enriched for nonsynonymous strain variation compared to other genes, suggesting a potential evolutionary benefit to maintaining strain diversity in specific genes. Finally, we show that p-crAssphage can be acquired through fecal microbiota transplantation.

中文翻译:


人类肠道定殖的粗样噬菌体的获取、传播和菌株多样性。



CrAss 样噬菌体是人类肠道微生物组中普遍存在的双链 DNA 病毒。在这里,我们分析了来自母婴对和接受粪便微生物群移植的患者的肠道宏基因组数据,以评估类crAss噬菌体的获取、传播和菌株多样性的模式。我们发现,类噬菌体在出生时很少被检测到,但在出生一个月后在婴儿微生物组中越来越普遍。我们在 50% 的病例中观察到几乎相同的基因组,在母亲和婴儿中都检测到相同的 crAss 样进化枝,这表明垂直传播。在假定原型 crAssphage (p-crAssphage) 传播的情况下,我们发现在婴儿中检测到了母亲中存在的一部分菌株,并且婴儿中的菌株多样性随着时间的推移而增加。与其他基因相比,假定的尾纤维蛋白因非同义菌株变异而富集,这表明维持特定基因的菌株多样性具有潜在的进化益处。最后,我们证明 p-crAssphage 可以通过粪便微生物群移植获得。
更新日期:2020-01-15
down
wechat
bug