Label-free fluorescence assay based on near-infrared B,N-doped carbon dots as a fluorescent probe for the detection of sialic acid,New Journal of Chemistry

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Label-free fluorescence assay based on near-infrared B,N-doped carbon dots as a fluorescent probe for the detection of sialic acid
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020/01/15 , DOI: 10.1039/c9nj05981d
Nan Wang _{1,

2,

3,

4,

5} , Mengke Wang _{1,

2,

3,

4,

5} , Yang Yu _{2,

3,

6,

7,

8} , Guojian Yang _{2,

3,

6,

7,

8} , Xingguang Su _{1,

2,

3,

4,

5}

Affiliation

Near-infrared fluorescent nanomaterials are highly promising materials for biomedical applications because of their avoidance of the auto-fluorescence of organisms. We herein report a facile one-pot synthesis of near-infrared B,N co-doped carbon dots (CDs) by using o-phenylenediamine (OPD) and 3-aminophenylboronic acid (ABPA) as precursors. The prepared near-infrared B,N-doped carbon dots with a fluorescence emission wavelength of 645 nm have abundant boric acid groups on the surface of the CDs that can combine with the ortho-dihydroxy group of sialic acid (SA) to form a five-membered ring, leading to the formation of a CD/SA complex, which can enhance the fluorescence intensity of the CDs. Thus a simple and sensitive fluorescence method for the detection of sialic acid was established. The fluorescence intensity change of the system (F/F₀) exhibited a good linear relationship with the concentration of sialic acid in the range of 20–1000 μM with a detection limit of 9.24 μM. This near-infrared fluorescent probe was successfully applied to the detection of sialic acid in simulating human saliva samples with satisfactory results.

中文翻译：

基于近红外B，N掺杂碳点的无标记荧光测定法，用于检测唾液酸

由于其避免了生物体的自发荧光，近红外荧光纳米材料是用于生物医学应用的高度有前途的材料。我们在本文中报道了通过使用邻苯二胺（OPD）和3-氨基苯基硼酸（ABPA）作为前体的近红外B，N共掺杂碳点（CDs）的简便一锅合成方法。制备的荧光发射波长为645 nm的近红外B，N掺杂碳点在CD的表面具有丰富的硼酸基团，可以与邻位结合唾液酸（SA）的-二羟基形成一个五元环，导致形成CD / SA复合物，从而可以增强CD的荧光强度。因此，建立了一种简单而灵敏的荧光方法检测唾液酸。系统的荧光强度变化（F / F ₀）与唾液酸浓度在20–1000μM范围内表现出良好的线性关系，检出限为9.24μM。该近红外荧光探针已成功应用于模拟唾液样品中唾液酸的检测，结果令人满意。

更新日期：2020-02-13

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