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Development of a Multiplex RT-qPCR for the Detection of Different Clades of Avian Influenza in Poultry.
Viruses ( IF 3.8 ) Pub Date : 2020-01-15 , DOI: 10.3390/v12010100 Tran Bac Le 1, 2 , Hye Kwon Kim 3 , Woonsung Na 4 , Van Phan Le 5 , Min-Suk Song 6 , Daesub Song 7 , Dae Gwin Jeong 1, 2 , Sun-Woo Yoon 1, 2
Viruses ( IF 3.8 ) Pub Date : 2020-01-15 , DOI: 10.3390/v12010100 Tran Bac Le 1, 2 , Hye Kwon Kim 3 , Woonsung Na 4 , Van Phan Le 5 , Min-Suk Song 6 , Daesub Song 7 , Dae Gwin Jeong 1, 2 , Sun-Woo Yoon 1, 2
Affiliation
Since the initial detection of H5N1, a highly pathogenic avian influenza (HPAI) virus, in 1996 in China, numerous HPAI H5 lineages have been classified, and they continue to pose a threat to animal and human health. In this study, we developed a novel primer/probe set that can be employed to simultaneously detect pan-H5 HPAI and two clades, 2.3.2.1 and 2.3.4.4, of H5Nx viruses using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The sensitivity and specificity of these primer sets and probes were confirmed with a number of different subtypes of influenza virus and the H5-HA gene plasmid DNA. In particular, the multiplex RT-qPCR assay was successfully applied to the simultaneous detection of H5 HPAI and different virus clades in clinical field samples from a poultry farm. Therefore, this multiplex assay and a novel detection primer set and probes will be useful for the laboratory diagnosis and epidemiological field studies of different circulating H5 HPAI virus clades in poultry and migratory wild birds.
更新日期：2020-01-15
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