To reduce phenolic pollutants in the environment, many countries have imposed firm restrictions on industrial wastewater discharge. In addition, the current industrial process of phenolic resin production uses phenol and formaldehyde as the reactants to perform a polycondensation reaction. Due to the toxicity of formaldehyde and phenolic pollutants, the main purpose of this research was to design a green process using horseradish peroxidase (HRP) enzymatic polymerization to remove phenols and to produce formaldehyde-free phenolic polymers. In this study, the optimal reaction conditions, such as reaction temperature, pH, initial phenol concentration and initial ratio of phenol, and H2O2, were examined. Then, the parameters of the enzyme kinetics were determined. To solve the restriction of enzyme inactivation, several nonionic surfactants were selected to improve the phenol removal efficiency, and the optimal operation conditions in a surfactant-containing system were also confirmed. Importantly, the molecular weight of the synthetic phenolic polymers could be controlled by adjusting the ratio of phenol and H2O2. The content of biphenols in the products was almost undetectable. Collectively, a green chemistry process was proposed in this study and would benefit the treatment of phenol-containing wastewater and the production of formaldehyde-free phenolic resin in the future.

中文翻译：

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开发一种含表面活性剂的方法，以提高辣根在水溶液中的去除效率并控制辣根过氧化物酶控制合成酚类聚合物的分子量。

为了减少环境中的酚类污染物，许多国家对工业废水的排放实行了严格的限制。另外，当前的酚醛树脂生产的工业过程使用苯酚和甲醛作为反应物来进行缩聚反应。由于甲醛和酚类污染物的毒性，本研究的主要目的是设计一种利用辣根过氧化物酶（HRP）酶促聚合去除酚类的绿色工艺，并生产不含甲醛的酚醛聚合物。在这项研究中，研究了最佳反应条件，例如反应温度，pH，初始苯酚浓度和初始苯酚比例以及H2O2。然后，确定酶动力学的参数。为了解决酶失活的限制，选择了几种非离子表面活性剂以提高苯酚的去除效率，并确定了在含表面活性剂的体系中的最佳操作条件。重要的是，可以通过调节苯酚和H 2 O 2的比例来控制合成酚醛聚合物的分子量。产品中双酚的含量几乎无法检测到。总体而言，本研究提出了一种绿色化学方法，该方法将有利于将来处理含酚废水和生产不含甲醛的酚醛树脂。产品中双酚的含量几乎无法检测到。总体而言，本研究提出了一种绿色化学方法，该方法将有利于将来处理含酚废水和生产不含甲醛的酚醛树脂。产品中双酚的含量几乎无法检测到。总体而言，本研究提出了一种绿色化学方法，该方法将有利于将来处理含酚废水和生产不含甲醛的酚醛树脂。