Bioremediation of environmental estrogens requires microorganisms with stable degradation efficiency and great stress tolerance in complex environments. In this work, Stenotrophomonas maltophilia SJTL3 isolated from wastewater was found to be able to degrade over 90% of 10 μg/mL 17β-estradiol (E2) in a week and the degradation dynamic was fitted by the first-order kinetic equations. Estrone was the first and major intermediate of E2 biodegradation. Strain SJTL3 exhibited strong tolerance to several adverse conditions like extreme pH (3.0-11.0), high osmolality (2%), co-existing heavy metals (6.25 μg/mL of Cu2+) and surfactants (5 CMC of Tween 80), and retained normal cell vitality and stable E2-degradaing efficiency. In solid soil, strain SJTL3 could remove nearly 100% of 1 μg/mL of E2 after the bacteria inoculation and 8-day culture. As to the contamination of 10 μg/mL E2 in soil, the biodegradation efficiency was about 90%. The further obtainment of the whole genome of strain SJTL3 and genome analysis revealed that this strain contained not only the potential genes responsible for estrogen degradation, but also the genes encoding proteins involved in stress tolerance. This work could promote the estrogen-biodegrading mechanism study and provide insights into the bioremediation application.

中文翻译：

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耐受有害环境因素的降解17β-雌二醇的嗜麦芽窄食单胞菌SJTL3的特征。

环境雌激素的生物修复要求微生物在复杂环境中具有稳定的降解效率和很大的抗逆性。在这项工作中，发现从废水中分离的嗜麦芽窄食单胞菌SJTL3能够在一周内降解10％/ g的17β-雌二醇（E2）的90％以上，并且降解动力学符合一阶动力学方程。雌酮是E2生物降解的第一个也是主要的中间体。SJTL3菌株对几种不利条件表现出较强的耐受力，例如极端pH（3.0-11.0），高渗透压（2％），重金属（6.25μg/ mL Cu2 +）和表面活性剂（Tween 80的5 CMC）共存并保留正常的细胞活力和稳定的E2降解效率。在固体土壤中，菌株SJTL3可以在细菌接种和8天培养后去除近100％的1μg/ mL E2。至于土壤中10μg/ mL E2的污染，其生物降解效率约为90％。SJTL3菌株全基因组的进一步获得和基因组分析表明，该菌株不仅包含负责雌激素降解的潜在基因，而且还包含编码抗逆性蛋白的基因。这项工作可以促进雌激素生物降解机制的研究，并为生物修复应用提供见识。