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Preparation and application of silica nanoparticles-Ocimum basilicum seeds bio-hybrid for the efficient immobilization of invertase enzyme.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-15 , DOI: 10.1016/j.colsurfb.2020.110796
Archana Mishra 1 , Jose Savio Melo 1 , Ashish Agrawal 2 , Yogesh Kashyap 2 , Debasis Sen 3
Affiliation  

For efficient utilization of immobilized invertase enzyme, a novel bio-hybrid support comprising silica nanoparticles and Ocimum basilicum seed was synthesized. Ocimum basilicum seeds provide a natural fibrouspellicular structure which acts as template for assembly of silica nanoparticles. Bio-hybrids of two different morphologies have been obtained by changing the physico-chemical conditions of the assembly process. Developed bio-hybrids were characterized through small angle X-ray scattering (SAXS), scanning electron microscopy (SEM), Synchrotron radiation based X-ray micro-computed tomography (SRμCT), Brunauer-Emmett-Teller (BET) and Fourier transform infrared spectroscopy (FTIR). Incorporation of the nanoparticles results to a fourfold increase in the available surface area of the seeds which is one of the important criteria for an immobilizing support. Synthesized bio-hybrids were used for the immobilization of commercially applicable invertase enzyme and efficient loading of enzyme was realized. Enzyme immobilized bio-hybrids could be easily separated out and reused up to eight times with 82 % retention of enzyme activity. Present work suggests that the unique features of the bio-hybrid make it suitable candidate for immobilization of enzymes in general.

中文翻译:

二氧化硅纳米粒子-罗勒种子生物杂化的制备和应用,用于高效固定转化酶。

为了有效利用固定化的转化酶,合成了一种新型的生物杂化载体,该载体包括二氧化硅纳米颗粒和罗勒属种子。罗勒罗勒种子提供了天然的纤维状针状结构,可作为二氧化硅纳米粒子组装的模板。通过改变组装过程的物理化学条件,已经获得了两种不同形态的生物杂交体。通过小角度X射线散射(SAXS),扫描电子显微镜(SEM),基于同步辐射的X射线微计算机断层扫描(SRμCT),Brunauer-Emmett-Teller(BET)和傅立叶变换红外光谱对已开发的生物杂交体进行表征光谱(FTIR)。纳米颗粒的掺入导致种子的可用表面积增加四倍,这是固定载体的重要标准之一。使用合成的生物杂交物固定可商购的转化酶,并实现了酶的有效负载。固定化酶的生物杂交物可以轻松分离出来,最多可重复使用八次,保留82%的酶活性。目前的工作表明,生物杂交体的独特特征使其通常适合固定化酶。固定化酶的生物杂交物可以轻松分离出来,最多可重复使用八次,保留82%的酶活性。目前的工作表明,生物杂交体的独特特征使其通常适合固定化酶。固定化酶的生物杂交物可以轻松分离出来,最多可重复使用八次,保留82%的酶活性。目前的工作表明,生物杂交体的独特特征使其通常适合固定化酶。
更新日期:2020-01-15
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