Abstract Different glucose-responsive nanogels of N-isopropylacrylamide (NIPAM) and 4-(1,6-dioxo-2,5-diaza-7-oxamyl) phenylboronic acid (DDOPBA) were synthesized using dextran-grafted maleic acid (Dex-MA) as a crosslinker. The formed nanogels (P(NIPAM-co-Dex-co-DDOPBA)s) were verified by 1H NMR, TEM, DLS and X-ray photoelectron spectroscopy (XPS). The incorporation of DDOPBA provided a remarkable sensitivity towards glucose in physiological pH due to the existence of electron-withdrawing group in its structure. Similarly, the hydrophilic Dex-MA modulated the temperature-sensitivity near physiological temperature. The nanogels exhibited high insulin loading capacity and encapsulation efficiency and the in vitro release profiles demonstrated a glucose dependant release of the payload at physiological pH and temperature.

中文翻译：

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在生理条件下具有自我调节胰岛素释放的葡聚糖交联葡萄糖响应纳米凝胶

摘要 采用葡聚糖接枝马来酸 (Dex-MA) 合成了 N-异丙基丙烯酰胺 (NIPAM) 和 4-(1,6-dioxo-2,5-diaza-7-oxamyl) 苯基硼酸 (DDOPBA) 的不同葡萄糖响应纳米凝胶。 ) 作为交联剂。形成的纳米凝胶 (P(NIPAM-co-Dex-co-DDOPBA)s) 通过 1H NMR、TEM、DLS 和 X 射线光电子能谱 (XPS) 进行验证。由于其结构中存在吸电子基团，DDOPBA的掺入对生理pH中的葡萄糖提供了显着的敏感性。类似地，亲水性 Dex-MA 调节接近生理温度的温度敏感性。纳米凝胶表现出高胰岛素负载能力和封装效率，体外释放曲线表明有效载荷在生理 pH 值和温度下的葡萄糖依赖性释放。