In September 2016, clinical signs, indicative of bluetongue, were observed in sheep in Cyprus. Bluetongue virus serotype 8 (BTV-8) was detected in sheep, indicating the first incursion of this serotype into Cyprus. Following virus propagation, Nextera XT DNA libraries were sequenced on the MiSeq instrument. Full-genome sequences were obtained for five isolates CYP2016/01-05 and the percent of nucleotide sequence (% nt) identity between them ranged from 99.92% to 99.95%, which corresponded to a few (2-5) amino acid changes. Based on the complete coding sequence, the Israeli ISR2008/13 (98.42-98.45%) was recognised as the closest relative to CYP2016/01-05. However, the phylogenetic reconstruction of CYP2016/01-05 revealed that the possibility of reassortment in several segments: 4, 7, 9 and 10. Based on the available sequencing data, the incursion BTV-8 into Cyprus most likely occurred from the neighbouring countries (e.g., Israel, Lebanon, Syria, or Jordan), where multiple BTV serotypes were co-circulating rather than from Europe (e.g., France) where a single BTV-8 serotype was dominant. Supporting this hypothesis, atmospheric dispersion modelling identified wind-transport events during July-September that could have allowed the introduction of BTV-8 infected midges from Lebanon, Syria or Israel coastlines into the Larnaca region of Cyprus.

中文翻译：

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2016 年 9 月塞浦路斯 8 型蓝舌病病毒爆发的起源。


2016 年 9 月，在塞浦路斯的绵羊身上观察到了蓝舌病的临床症状。在绵羊中检测到蓝舌病毒血清型 8 (BTV-8)，表明该血清型首次入侵塞浦路斯。病毒传播后，Nextera XT DNA 文库在 MiSeq 仪器上进行测序。获得了 5 个分离株 CYP2016/01-05 的全基因组序列，它们之间的核苷酸序列百分比 (% nt) 同一性范围为 99.92% 至 99.95%，这对应于一些 (2-5) 个氨基酸变化。基于完整的编码序列，以色列ISR2008/13（98.42-98.45%）被认为是与CYP2016/01-05最接近的亲戚。然而，CYP2016/01-05的系统发育重建揭示了几个片段重配的可能性：4、7、9和10。根据现有的测序数据，BTV-8入侵塞浦路斯最有可能来自邻国（例如以色列、黎巴嫩、叙利亚或约旦），多个 BTV 血清型共同传播，而不是来自欧洲（例如法国），其中单一 BTV-8 血清型占主导地位。支持这一假设的是，大气扩散模型确定了 7 月至 9 月期间的风输送事件，这些事件可能导致受 BTV-8 感染的蠓从黎巴嫩、叙利亚或以色列海岸线引入塞浦路斯拉纳卡地区。