Yarrowia lipolytica is a well-known oleaginous yeast that naturally accumulates lipids to more than 20% of their dry cell weight. Due to its brief doubling time and Generally Recognized as Safe (GRAS) properties, Y. lipolytica has been exploited for the production of commercially valuable lipids. Among the genes related to the lipid synthesis, the gene YALI0E16797g (LRO1) encoding a major triacylglycerol synthase of Y. lipolytica shows a significant impact during the acylation process. Thus, in the present work, we explore the contributions of hp4d or TEFintron promoters to the response of LRO1 expression on lipid accumulation by molecular cloning technology. Results showed that over-expression of LRO1 led to higher lipid content as well as lipid yield. The one with the hp4d promoter showed the highest lipid content of 12% wt. However, such an enhancement also caused a growth defect of cells. On the other hand, the lipid content of the cells over-expressing LRO1 with TEFintron promoter revealed only a modest increase in lipid content, but it promoted cell growth. Therefore, all things considered the one with the TEFintron promoter showed the highest lipid yield.

中文翻译：

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在两个不同的启动子下过表达lro1基因增强了解脂耶氏酵母Po1g中脂质的产生。

解脂耶氏酵母（Yarrowia lipolytica）是一种众所周知的含油酵母，其天然积累脂质至干细胞重量的20％以上。由于其短暂的加倍时间和公认的安全（GRAS）特性，解脂耶氏酵母已被用于生产具有商业价值的脂质。在与脂质合成有关的基因中，编码解脂耶氏酵母的主要三酰基甘油合酶的基因YALI0E16797g（LRO1）在酰化过程中显示出重大影响。因此，在本研究中，我们通过分子克隆技术探索了hp4d或TEFintron启动子对LRO1表达对脂质积累的响应的贡献。结果表明，LRO1的过表达导致脂质含量更高和脂质产量更高。带有hp4d启动子的蛋白显示最高脂质含量为12％wt。然而，这种增强还引起细胞的生长缺陷。另一方面，用TEFintron启动子过表达LRO1的细胞的脂质含量仅显示脂质含量的适度增加，但促进细胞生长。因此，所有认为具有TEFintron启动子的事物都显示出最高的脂质产量。