BACKGROUND Recurrent aphthous stomatitis (RAS) is a common oral disease with unknown molecular pathogenesis. Our preliminary microarray analysis revealed the altered expression of lncRNA Cancer Susceptibility Gene 2 (CASC2) in RAS. We therefore analyzed the role of CASC2 in RAS. METHODS In this study, plasma samples were obtained from RAS patients and healthy participants. Plasma levels of CASC2 were measured by RT-qPCR. Plasma levels of IL-6 and IL-18 were measured by enzyme-linked immunosorbent assay (ELISA). A follow-up study was performed to analyze the role of CASC2 in the recurrence of RAS. RESULTS In the present study, we found that lncRNA Cancer Susceptibility Gene 2 (CASC2), as well as pro-inflammatory factors interleukin 6 (IL-6) and interleukin 18 (IL-18), were upregulated in plasma of RAS patients compared with healthy participants. Plasma levels of lncRNA CASC2 were positively correlated with plasma levels of IL-6 and IL-18 in RAS patients but not in healthy participants. Compared with pre-treatment levels, plasma levels of lncRNA CASC2, IL-6 and IL-18 were reduced after recovery. A follow-up study showed that patients with high levels of lncRNA CASC2 had a significantly higher recurrence rate. CONCLUSION LncRNA CASC 2 is upregulated in RAS and predicts the recurrence.

中文翻译：

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LncRNA CASC 2在口疮性口炎中上调，并预测其复发。

背景技术复发性口疮性口炎（RAS）是一种具有未知分子发病机制的常见口腔疾病。我们的初步芯片分析揭示了RAS中lncRNA癌症易感基因2（CASC2）的表达改变。因此，我们分析了CASC2在RAS中的作用。方法在本研究中，血浆样本来自RAS患者和健康参与者。通过RT-qPCR测量CASC2的血浆水平。通过酶联免疫吸附测定（ELISA）测量血浆IL-6和IL-18水平。进行了一项后续研究，以分析CASC2在RAS复发中的作用。结果在本研究中，我们发现RAS患者血浆中的lncRNA癌症易感基因2（CASC2）以及促炎因子白细胞介素6（IL-6）和白细胞介素18（IL-18）被上调。健康的参与者。在RAS患者中，lncRNA CASC2的血浆水平与IL-6和IL-18的血浆水平呈正相关，而在健康受试者中则没有。与治疗前水平相比，恢复后lncRNA CASC2，IL-6和IL-18的血浆水平降低。一项后续研究表明，lncRNA CASC2水平高的患者复发率明显更高。结论LncRNA CASC 2在RAS中上调，并预测其复发。