BACKGROUND In recent decades, there has been a marked increase in the number of reported cases of pertussis around the world, and pertussis continues to be a frequently occurring disease despite an effective childhood vaccination. This study aims to determine the role of household contacts of children diagnosed with pertussis in Casablanca Morocco. METHODS From November 2015 to October 2017, children suspected of whooping cough that consulted Ibn Rochd University hospital at Casablanca with their household contacts were enrolled in the study. Nasopharyngeal (NP) samples of the suspected children were analyzed by culture and RT-PCR. For the household contacts, NP and blood samples were collected and analyzed by RT-PCR and specific detection of pertussis toxin antibodies by ELISA, respectively. RESULTS During the study period, the survey was carried out on 128 infants hospitalized for pertussis suspicion and their families (N = 140). B. pertussis DNA was specifically detected in 73 (57%) samples, coexistence of B. pertussis and B. parapertussis DNA in 3 (2.3%) samples, coexistence of B. pertussis and B. holmesii DNA in 10 (7.81%) and only one (0.78%) sample was IS 481 RT-PCR positive without the possibility of determining the Bordetella species with the diagnostic tools used. Confirmations of Pertussis infection in household contacts by culture, RT- PCR and serology were 10, 46 and 39%, respectively. B. pertussis DNA was confirmed in the infants as well in their mothers in 38% of the cases. Co detection of B. pertussis and B. parapertussis DNA in 2% and co-detection of B. pertussis and B. holmesii DNA in 4%. B. holmesii DNA alone was detected in 5 NP samples of index cases and their mothers. CONCLUSIONS The results of this study confirm that B. pertussis is still circulating in children and adults, and were likely a source of pertussis contamination in infants still not vaccinated. The use of RT-PCR specific for B. pertussis in the diagnosis of adults is less sensitive and should be associated with serologic tests to improve diagnosis of pertussis and contributes to preventing transmission of the disease in infants.

中文翻译：

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摩洛哥卡萨布兰卡的婴儿、母亲和其他接触者中存在百日咳。


背景技术近几十年来，世界各地报告的百日咳病例数量显着增加，尽管儿童接种了有效的疫苗，但百日咳仍然是一种常见疾病。本研究旨在确定摩洛哥卡萨布兰卡被诊断患有百日咳的儿童的家庭接触者的作用。方法 2015年11月至2017年10月，在卡萨布兰卡伊本·罗赫德大学医院就诊的疑似百日咳儿童及其家庭接触者被纳入研究。对疑似儿童的鼻咽（NP）样本进行了培养和RT-PCR分析。对于家庭接触者，采集NP和血样，分别采用RT-PCR进行分析，并采用ELISA对百日咳毒素抗体进行特异性检测。结果研究期间，对128名疑似百日咳住院的婴儿及其家属进行了调查（N = 140）。在 73 个（57%）样本中特异检测到百日咳博德特氏菌 DNA，在 3 个（2.3%）样本中检测到百日咳博德特氏菌和副百日咳博德特氏菌 DNA 共存，在 10 个（7.81%）样本中检测到百日咳博德特氏菌和霍姆斯博德特氏菌 DNA 共存，只有一份 (0.78%) 样本 IS 481 RT-PCR 呈阳性，无法使用所使用的诊断工具确定博德特氏菌属。通过培养、RT-PCR 和血清学检测，家庭接触者中百日咳感染的确诊率分别为 10%、46% 和 39%。 38% 的病例中，婴儿及其母亲体内均检测到百日咳博德特氏菌 DNA。联合检测 2% 百日咳博德特氏菌和副百日咳博德特氏菌 DNA，联合检测 4% 百日咳博德特氏菌和霍姆斯博德特氏菌 DNA。在指示病例及其母亲的 5 个 NP 样本中仅检测到 B. holmesii DNA。结论 本研究结果证实 B. 百日咳仍在儿童和成人中流行，并且可能是尚未接种疫苗的婴儿的百日咳污染源。使用百日咳博德特氏菌特异性 RT-PCR 诊断成人的敏感性较低，应与血清学检测相结合，以改善百日咳的诊断并有助于预防婴儿中的疾病传播。