Abstract Highly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems.

中文翻译：

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银汞合金颗粒上牛血清白蛋白的恒流计时电位剥离检测

摘要 通过恒流计时电位剥离 (CPS) 分析记录的高灵敏度峰值 H 与吸附在汞基表面上的蛋白质的催化析氢反应有关，包括最近引入的银汞合金颗粒 (AgAP)。由于峰值 H 允许对蛋白质进行结构敏感分析，包括那些在生物医学和癌症研究中重要的蛋白质，因此在这项工作中使用 AgAP 通过 CPS 分析系统地研究了它。AgAPs 在基面热解石墨电极 (bPGE) 上的电沉积条件针对使用峰值 H 的模型蛋白（牛血清白蛋白，BSA）的 CPS 分析进行了优化。 bPGE-AgAP 制备的优化条件允许对天然或变性 BSA 进行灵敏的 CPS 检测低至 10 nM 浓度，区分两种形式，和 BSA 热变性动力学的记录。获得的结果证实了 AgAP 在蛋白质电分析中的适用性，并可能有助于使用微量批量、流通或芯片实验室检测系统进一步开发新型（生物）传感器或（生物）电化学应用。