A novel composite antimicrobial film (CAF), made from a pullulan-based biopolymer and polyethylene (PE) was developed and evaluated for controlling pathogens associated with muscle foods. Initially, CAFs were developed by incorporating thymol (T), nisin (N) and/or lauric arginate (LAE) into the pullulan layer and layering it on top of PE. The antimicrobial activity of the resulting CAFs was evaluated against cocktails of Shiga toxin-producing E. coli (STEC), Salmonella spp., Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus (S. aureus) in disk diffusion assays (DDAs). CAFs containing N were ineffective, while those containing T were effective for inhibiting the pathogens in DDAs. However, CAFs made with them did not exhibit desirable physical and mechanical properties since solvents (HCl and ethanol, respectively) interfered with the binding of pullulan to PE. Conversely, CAFs made with 0.5, 1 and 2.5% LAE maintained proper physical and mechanical characteristics and inhibited the four bacterial pathogens in DDAs. Based on these preliminary results, cocktails consisting of approximately 8 log10 CFU/ml of STEC, Salmonella, L. monocytogenes, or S. aureus were experimentally-inoculated onto raw beef, raw chicken breast, or ready-to-eat (RTE) turkey breast to obtain approximately 6.6 log10 CFU/cm2, aseptically transferred to CAFs containing 0.5, 1, or 2.5% LAE that were made into sachets/bags, vacuum packaged, sealed, and remaining microbial populations determined up to 28 days of refrigerated storage (4 °C). By day 28, CAFs containing 0.5, 1, and 2.5% LAE reduced: STEC by 1.13, 1.33 and 2.88 log10 CFU/cm2 respectively, on raw beef; Salmonella by 2.03, 2.12 and 3.01 log10 CFU/cm2 respectively, on raw chicken breast; L. monocytogenes by 1.12, 1.81 and 3.56 log10 CFU/cm2 respectively, on RTE turkey breast; and S. aureus by 0.68, 2.02 and 3.43 log10 CFU/cm2, respectively, on RTE turkey breast. CAFs may be of interest to the meat and poultry industry to control foodborne pathogens associated with these food products.

中文翻译：

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与乳链菌肽，百里香酚和月桂酸精氨酸结合使用的支链淀粉基复合抗菌膜（CAF）的开发和评估，可减少与肌肉食品相关的食源性病原体。

由基于支链淀粉的生物聚合物和聚乙烯（PE）制成的新型复合抗菌膜（CAF）已开发并进行了评估，以控制与肌肉食品相关的病原体。最初，通过将百里香酚（T），乳链菌肽（N）和/或月桂酸月桂酸酯（LAE）掺入支链淀粉层并将其分层到PE上来开发CAF。在圆盘扩散测定法（DDAs）中，评估了产生的CAFs对产志贺毒素的大肠杆菌（STEC），沙门氏菌，单核细胞增生性李斯特菌（L. monocytogenes）和金黄色葡萄球菌（S. aureus）的混合物的抗菌活性。含有N的CAF无效，而含有T的CAF有效抑制DDA中的病原体。但是，用它们制成的CAF并没有表现出理想的物理和机械性能，因为溶剂（HCl和乙醇，分别）干扰了支链淀粉与PE的结合。相反，用0.5％，1％和2.5％LAE制成的CAF保持适当的物理和机械特性，并抑制了DDA中的四种细菌病原体。根据这些初步结果，将由大约8 log10 CFU / ml的STEC，沙门氏菌，单核细胞增生李斯特氏菌或金黄色葡萄球菌组成的混合物通过实验接种到生牛肉，生鸡胸肉或即食（RTE）火鸡上乳房获得大约6.6 log10 CFU / cm2，无菌转移到包含0.5％，1％或2.5％LAE的CAF中，制成小袋/袋，真空包装，密封，并在冷藏保存28天后确定剩余微生物种群（4 °C）。到第28天，包含0.5％，1％和2.5％LAE的CAF减少了：生牛肉的STEC分别降低了1.13、1.33和2.88 log10 CFU / cm2。生鸡肉胸腺沙门氏菌分别降低2.03、2.12和3.01 log10 CFU / cm2; 在RTE火鸡胸上单核细胞增生李斯特菌分别以1.12、1.81和3.56 log10 CFU / cm2记录; RTE火鸡胸上的金黄色葡萄球菌和金黄色葡萄球菌分别降低0.68、2.02和3.43 log10 CFU / cm2。CAF可能对肉类和家禽业感兴趣，以控制与这些食品相关的食源性病原体。