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Comparative histology of wild-type and p53-deficient medaka (Oryzias latipes): nephrotoxic effect of ultraviolet A radiation.
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020-01-29 , DOI: 10.1039/c9pp00236g Alaa El-Din H Sayed 1 , Shaimaa M M Saleh , Hiroshi Mitani
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020-01-29 , DOI: 10.1039/c9pp00236g Alaa El-Din H Sayed 1 , Shaimaa M M Saleh , Hiroshi Mitani
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Ultraviolet radiation is an ecological factor that directly affects terrestrial organisms through suppression of immunity or damage to internal organs. The present study assessed the effects of ultraviolet A (UVA) radiation on the kidneys of both wild-type (WT) and p53-deficient medaka (Oryzias latipes) and evaluated which strain was more resistant to the effects of UVA. Fish were divided into four groups: control group 1 (Cwt and Cp53), kept for 3 days without UVA exposure; group 2 (1wt and 1p53), fish exposed daily to UVA for 1 h day-1 for 3 days; group 3 (2wt and 2p53), fish exposed daily to UVA for 2 h day-1 for 3 days; and group 4 (3wt and 3p53), fish exposed daily to UVA for 3 h day-1 for 3 days. Samples of tissues were obtained 24 h after UVA exposure. The most obvious histopathological changes induced by UVA radiation in kidney tissues of both strains of medaka (WT and p53-deficient) were high levels of vacuolation of tubular cells followed by necrosis. The tubular segments lost their normal shape which appeared like a network structure and their cells with clear cytoplasm. Necrosis of lymphoid tissues and spots of brown pigmentation (possibly melanomacrophages) were sporadically seen in interstitial lymphoid tissues, while shrinkage of glomeruli, diminution of periodic acid-Schiff staining, and increased amount of collagenous fibers were observed. Our results confirmed the harmful effects of UVA radiation on kidney tissues of both WT and p53-deficient medaka. However, WT medaka was affected more than p53-deficient medaka.
中文翻译：
野生型和p53缺乏型高加索（Oryzias latipes）的比较组织学：紫外线A辐射的肾毒性作用。
紫外线辐射是一种生态因子，可通过抑制免疫力或破坏内部器官而直接影响陆地生物。本研究评估了紫外线A（UVA）辐射对野生型（WT）和p53缺陷型高加索（Oryzias latipes）肾脏的影响，并评估了哪种菌株对UVA的耐受性更高。将鱼分为四组：对照组1（Cwt和Cp53），保持3天不暴露于UVA；第2组（1wt和1p53），每天将鱼暴露于UVA中1天-1天3天；第3组（2wt和2p53），每天将鱼暴露于UVA中2天-1天，持续3天；和第4组（3wt和3p53），每天将鱼暴露于UVA中3天-1天，持续3天。暴露于UVA 24小时后获得组织样品。在两个高加索菌株（WT和p53缺陷型）的肾脏组织中，UVA辐射引起的最明显的组织病理学改变是肾小管细胞的空泡化程度高，随后坏死。管状节段失去其正常形状，看起来像网络结构，其细胞具有清晰的细胞质。在间质性淋巴组织中偶尔可见淋巴样组织坏死和棕色色素沉着斑（可能是黑色素巨噬细胞），同时观察到肾小球萎缩，高碘酸-希夫染色减少和胶原纤维增多。我们的结果证实了UVA辐射对野生型和p53缺乏型medaka肾脏组织的有害影响。但是，WT medaka受到的影响比p53缺乏的medaka多。
更新日期：2020-02-19
中文翻译：
野生型和p53缺乏型高加索（Oryzias latipes）的比较组织学：紫外线A辐射的肾毒性作用。
紫外线辐射是一种生态因子，可通过抑制免疫力或破坏内部器官而直接影响陆地生物。本研究评估了紫外线A（UVA）辐射对野生型（WT）和p53缺陷型高加索（Oryzias latipes）肾脏的影响，并评估了哪种菌株对UVA的耐受性更高。将鱼分为四组：对照组1（Cwt和Cp53），保持3天不暴露于UVA；第2组（1wt和1p53），每天将鱼暴露于UVA中1天-1天3天；第3组（2wt和2p53），每天将鱼暴露于UVA中2天-1天，持续3天；和第4组（3wt和3p53），每天将鱼暴露于UVA中3天-1天，持续3天。暴露于UVA 24小时后获得组织样品。在两个高加索菌株（WT和p53缺陷型）的肾脏组织中，UVA辐射引起的最明显的组织病理学改变是肾小管细胞的空泡化程度高，随后坏死。管状节段失去其正常形状，看起来像网络结构，其细胞具有清晰的细胞质。在间质性淋巴组织中偶尔可见淋巴样组织坏死和棕色色素沉着斑（可能是黑色素巨噬细胞），同时观察到肾小球萎缩，高碘酸-希夫染色减少和胶原纤维增多。我们的结果证实了UVA辐射对野生型和p53缺乏型medaka肾脏组织的有害影响。但是，WT medaka受到的影响比p53缺乏的medaka多。
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