HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics.,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-01-14 , DOI: 10.1021/acs.jproteome.9b00686
Dylan M Marchione ₁ , Ilyana Ilieva ₂ , Kyle Devins ₂ , Danielle Sharpe ₂ , Darryl J Pappin _{3,

4} , Benjamin A Garcia ₁ , John P Wilson ₄ , John B Wojcik ₂

Affiliation

Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.

中文翻译：

HYPERsol：来自用于临床蛋白质组学的存档 FFPE 组织的高质量数据。

世界各地都有大量福尔马林固定石蜡包埋 (FFPE) 组织档案，是临床蛋白质组学研究的宝贵资源。然而，FFPE 蛋白质组学的当前协议缺乏标准化、效率、再现性和可扩展性。在这里，我们介绍了通过直接增溶 (HYPERsol) 进行的高产蛋白质提取和回收，这是一种使用超声波处理和 S-Trap 样品处理的优化工作流程，可以使 FFPE 样品的蛋白质组覆盖和量化与快速冷冻组织的蛋白质组覆盖和定量相当（平均 R = 0.936）。当应用于档案样本时，HYPERsol 可从保存长达 17 年的 FFPE 标本中获得高质量数据，并且可能有助于发现新的免疫组织化学标记。

更新日期：2020-01-15

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11